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Zika virus RNA structure controls its unique neurotropism by bipartite binding to Musashi-1

Author

Listed:
  • Xiang Chen

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Yan Wang

    (Tsinghua University)

  • Zhonghe Xu

    (Tsinghua University)

  • Meng-Li Cheng

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Qing-Qing Ma

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Rui-Ting Li

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Zheng-Jian Wang

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Hui Zhao

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Xiaobing Zuo

    (X-ray Science Division, Argonne National Laboratory)

  • Xiao-Feng Li

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Xianyang Fang

    (Tsinghua University
    Chinese Academy of Sciences)

  • Cheng-Feng Qin

    (Beijing Institute of Microbiology and Epidemiology, AMMS
    Chinese Academy of Medical Sciences)

Abstract

Human RNA binding protein Musashi-1 (MSI1) plays a critical role in neural progenitor cells (NPCs) by binding to various host RNA transcripts. The canonical MSI1 binding site (MBS), A/GU(1-3)AG single-strand motif, is present in many RNA virus genomes, but only Zika virus (ZIKV) genome has been demonstrated to bind MSI1. Herein, we identified the AUAG motif and the AGAA tetraloop in the Xrn1-resistant RNA 2 (xrRNA2) as the canonical and non-canonical MBS, respectively, and both are crucial for ZIKV neurotropism. More importantly, the unique AGNN-type tetraloop is evolutionally conserved, and distinguishes ZIKV from other known viruses with putative MBSs. Integrated structural analysis showed that MSI1 binds to the AUAG motif and AGAA tetraloop of ZIKV in a bipartite fashion. Thus, our results not only identified an unusual viral RNA structure responsible for MSI recognition, but also revealed a role for the highly structured xrRNA in controlling viral neurotropism.

Suggested Citation

  • Xiang Chen & Yan Wang & Zhonghe Xu & Meng-Li Cheng & Qing-Qing Ma & Rui-Ting Li & Zheng-Jian Wang & Hui Zhao & Xiaobing Zuo & Xiao-Feng Li & Xianyang Fang & Cheng-Feng Qin, 2023. "Zika virus RNA structure controls its unique neurotropism by bipartite binding to Musashi-1," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36838-w
    DOI: 10.1038/s41467-023-36838-w
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    Cited by:

    1. Charlotte Lefèvre & Georgia M. Cook & Adam M. Dinan & Shiho Torii & Hazel Stewart & George Gibbons & Alex S. Nicholson & Liliana Echavarría-Consuegra & Luke W. Meredith & Valeria Lulla & Naomi McGover, 2024. "Zika viruses encode 5′ upstream open reading frames affecting infection of human brain cells," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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