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Zika virus RNA structure controls its unique neurotropism by bipartite binding to Musashi-1

Author

Listed:
  • Xiang Chen

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Yan Wang

    (Tsinghua University)

  • Zhonghe Xu

    (Tsinghua University)

  • Meng-Li Cheng

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Qing-Qing Ma

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Rui-Ting Li

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Zheng-Jian Wang

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Hui Zhao

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Xiaobing Zuo

    (X-ray Science Division, Argonne National Laboratory)

  • Xiao-Feng Li

    (Beijing Institute of Microbiology and Epidemiology, AMMS)

  • Xianyang Fang

    (Tsinghua University
    Chinese Academy of Sciences)

  • Cheng-Feng Qin

    (Beijing Institute of Microbiology and Epidemiology, AMMS
    Chinese Academy of Medical Sciences)

Abstract

Human RNA binding protein Musashi-1 (MSI1) plays a critical role in neural progenitor cells (NPCs) by binding to various host RNA transcripts. The canonical MSI1 binding site (MBS), A/GU(1-3)AG single-strand motif, is present in many RNA virus genomes, but only Zika virus (ZIKV) genome has been demonstrated to bind MSI1. Herein, we identified the AUAG motif and the AGAA tetraloop in the Xrn1-resistant RNA 2 (xrRNA2) as the canonical and non-canonical MBS, respectively, and both are crucial for ZIKV neurotropism. More importantly, the unique AGNN-type tetraloop is evolutionally conserved, and distinguishes ZIKV from other known viruses with putative MBSs. Integrated structural analysis showed that MSI1 binds to the AUAG motif and AGAA tetraloop of ZIKV in a bipartite fashion. Thus, our results not only identified an unusual viral RNA structure responsible for MSI recognition, but also revealed a role for the highly structured xrRNA in controlling viral neurotropism.

Suggested Citation

  • Xiang Chen & Yan Wang & Zhonghe Xu & Meng-Li Cheng & Qing-Qing Ma & Rui-Ting Li & Zheng-Jian Wang & Hui Zhao & Xiaobing Zuo & Xiao-Feng Li & Xianyang Fang & Cheng-Feng Qin, 2023. "Zika virus RNA structure controls its unique neurotropism by bipartite binding to Musashi-1," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36838-w
    DOI: 10.1038/s41467-023-36838-w
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    References listed on IDEAS

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    1. Robert P. Rambo & John A. Tainer, 2013. "Accurate assessment of mass, models and resolution by small-angle scattering," Nature, Nature, vol. 496(7446), pages 477-481, April.
    2. Andrii Slonchak & Leon E. Hugo & Morgan E. Freney & Sonja Hall-Mendelin & Alberto A. Amarilla & Francisco J. Torres & Yin Xiang Setoh & Nias Y. G. Peng & Julian D. J. Sng & Roy A. Hall & Andrew F. van, 2020. "Zika virus noncoding RNA suppresses apoptosis and is required for virus transmission by mosquitoes," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    3. Xiaolin Niu & Ruirui Sun & Zhifeng Chen & Yirong Yao & Xiaobing Zuo & Chunlai Chen & Xianyang Fang, 2021. "Pseudoknot length modulates the folding, conformational dynamics, and robustness of Xrn1 resistance of flaviviral xrRNAs," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    4. Masataka Okabe & Takao Imai & Mitsuhiko Kurusu & Yasushi Hiromi & Hideyuki Okano, 2001. "Translational repression determines a neuronal potential in Drosophila asymmetric cell division," Nature, Nature, vol. 411(6833), pages 94-98, May.
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