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Single-cell transcriptome profiling of the stepwise progression of head and neck cancer

Author

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  • Ji-Hye Choi

    (Ajou University School of Medicine
    Ajou University)

  • Bok-Soon Lee

    (Ajou University School of Medicine)

  • Jeon Yeob Jang

    (Ajou University
    Ajou University School of Medicine)

  • Yun Sang Lee

    (Ajou University School of Medicine)

  • Hyo Jeong Kim

    (Ajou University School of Medicine
    Ajou University)

  • Jin Roh

    (Ajou University School of Medicine)

  • Yoo Seob Shin

    (Ajou University School of Medicine)

  • Hyun Goo Woo

    (Ajou University School of Medicine
    Ajou University)

  • Chul-Ho Kim

    (Ajou University School of Medicine
    Ajou University School of Medicine)

Abstract

Head and neck squamous cell carcinoma (HNSCC) undergoes stepwise progression from normal tissues to precancerous leukoplakia, primary HNSCC, and metastasized tumors. To delineate the heterogeneity of tumor cells and their interactions during the progression of HNSCC, we employ single-cell RNA-seq profiling for normal to metastasized tumors. We can identify the carcinoma in situ cells in leukoplakia lesions that are not detected by pathological examination. In addition, we identify the cell type subsets of the Galectin 7B (LGALS7B)-expressing malignant cells and CXCL8-expressing fibroblasts, demonstrating that their abundance in tumor tissue is associated with unfavorable prognostic outcomes. We also demonstrate the interdependent ligand-receptor interaction of COL1A1 and CD44 between fibroblasts and malignant cells, facilitating HNSCC progression. Furthermore, we report that the regulatory T cells in leukoplakia and HNSCC tissues express LAIR2, providing a favorable environment for tumor growth. Taken together, our results update the pathobiological insights into cell-cell interactions during the stepwise progression of HNSCCs.

Suggested Citation

  • Ji-Hye Choi & Bok-Soon Lee & Jeon Yeob Jang & Yun Sang Lee & Hyo Jeong Kim & Jin Roh & Yoo Seob Shin & Hyun Goo Woo & Chul-Ho Kim, 2023. "Single-cell transcriptome profiling of the stepwise progression of head and neck cancer," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36691-x
    DOI: 10.1038/s41467-023-36691-x
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    References listed on IDEAS

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    1. Junyue Cao & Malte Spielmann & Xiaojie Qiu & Xingfan Huang & Daniel M. Ibrahim & Andrew J. Hill & Fan Zhang & Stefan Mundlos & Lena Christiansen & Frank J. Steemers & Cole Trapnell & Jay Shendure, 2019. "The single-cell transcriptional landscape of mammalian organogenesis," Nature, Nature, vol. 566(7745), pages 496-502, February.
    2. Hyun Goo Woo & Ji-Hye Choi & Sarah Yoon & Byul A. Jee & Eun Ju Cho & Jeong-Hoon Lee & Su Jong Yu & Jung-Hwan Yoon & Nam-Joon Yi & Kwang-Woong Lee & Kyung-Suk Suh & Yoon Jun Kim, 2017. "Integrative analysis of genomic and epigenomic regulation of the transcriptome in liver cancer," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
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    1. Z. L. Liu & X. Y. Meng & R. J. Bao & M. Y. Shen & J. J. Sun & W. D. Chen & F. Liu & Y. He, 2024. "Single cell deciphering of progression trajectories of the tumor ecosystem in head and neck cancer," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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