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Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto’s thyroiditis

Author

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  • Qian-Yue Zhang

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Xiao-Ping Ye

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Zheng Zhou

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
    the First Affiliated Hospital of Zhengzhou University)

  • Chen-Fang Zhu

    (Shanghai Jiao Tong University)

  • Rui Li

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Ya Fang

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Rui-Jia Zhang

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Lu Li

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Wei Liu

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Zheng Wang

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Shi-Yang Song

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Sang-Yu Lu

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Shuang-Xia Zhao

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Jian-Nan Lin

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Huai-Dong Song

    (Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
    Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

Abstract

Hashimoto’s thyroiditis (HT) is the most common autoimmune disease characterized by lymphocytic infiltration and thyrocyte destruction. Dissection of the interaction between the thyroidal stromal microenvironment and the infiltrating immune cells might lead to a better understanding of HT pathogenesis. Here we show, using single-cell RNA-sequencing, that three thyroidal stromal cell subsets, ACKR1+ endothelial cells and CCL21+ myofibroblasts and CCL21+ fibroblasts, contribute to the thyroidal tissue microenvironment in HT. These cell types occupy distinct histological locations within the thyroid gland. Our experiments suggest that they might facilitate lymphocyte trafficking from the blood to thyroid tissues, and T cell zone CCL21+ fibroblasts may also promote the formation of tertiary lymphoid organs characteristic to HT. Our study also demonstrates the presence of inflammatory macrophages and dendritic cells expressing high levels of IL-1β in the thyroid, which may contribute to thyrocyte destruction in HT patients. Our findings thus provide a deeper insight into the cellular interactions that might prompt the pathogenesis of HT.

Suggested Citation

  • Qian-Yue Zhang & Xiao-Ping Ye & Zheng Zhou & Chen-Fang Zhu & Rui Li & Ya Fang & Rui-Jia Zhang & Lu Li & Wei Liu & Zheng Wang & Shi-Yang Song & Sang-Yu Lu & Shuang-Xia Zhao & Jian-Nan Lin & Huai-Dong S, 2022. "Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto’s thyroiditis," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28120-2
    DOI: 10.1038/s41467-022-28120-2
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    References listed on IDEAS

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