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The ULK3 kinase is a determinant of keratinocyte self-renewal and tumorigenesis targeting the arginine methylome

Author

Listed:
  • Sandro Goruppi

    (Massachusetts General Hospital
    Massachusetts General Hospital and Harvard Medical School)

  • Andrea Clocchiatti

    (Massachusetts General Hospital
    Massachusetts General Hospital and Harvard Medical School)

  • Giulia Bottoni

    (Massachusetts General Hospital
    Massachusetts General Hospital and Harvard Medical School)

  • Emery Cicco

    (Massachusetts General Hospital
    Massachusetts General Hospital and Harvard Medical School)

  • Min Ma

    (Centre Hospitalier Universitaire Vaudois
    University of Lausanne)

  • Beatrice Tassone

    (Centre Hospitalier Universitaire Vaudois
    University of Lausanne)

  • Victor Neel

    (Massachusetts General Hospital and Harvard Medical School)

  • Shadhmer Demehri

    (Massachusetts General Hospital
    Massachusetts General Hospital and Harvard Medical School)

  • Christian Simon

    (Centre Hospitalier Universitaire Vaudois
    University of Lausanne
    International Cancer Prevention Institute)

  • G. Paolo Dotto

    (Massachusetts General Hospital
    Massachusetts General Hospital and Harvard Medical School
    Centre Hospitalier Universitaire Vaudois
    University of Lausanne)

Abstract

Epigenetic mechanisms oversee epidermal homeostasis and oncogenesis. The identification of kinases controlling these processes has direct therapeutic implications. We show that ULK3 is a nuclear kinase with elevated expression levels in squamous cell carcinomas (SCCs) arising in multiple body sites, including skin and Head/Neck. ULK3 loss by gene silencing or deletion reduces proliferation and clonogenicity of human keratinocytes and SCC-derived cells and affects transcription impinging on stem cell-related and metabolism programs. Mechanistically, ULK3 directly binds and regulates the activity of two histone arginine methyltransferases, PRMT1 and PRMT5 (PRMT1/5), with ULK3 loss compromising PRMT1/5 chromatin association to specific genes and overall methylation of histone H4, a shared target of these enzymes. These findings are of translational significance, as downmodulating ULK3 by RNA interference or locked antisense nucleic acids (LNAs) blunts the proliferation and tumorigenic potential of SCC cells and promotes differentiation in two orthotopic models of skin cancer.

Suggested Citation

  • Sandro Goruppi & Andrea Clocchiatti & Giulia Bottoni & Emery Cicco & Min Ma & Beatrice Tassone & Victor Neel & Shadhmer Demehri & Christian Simon & G. Paolo Dotto, 2023. "The ULK3 kinase is a determinant of keratinocyte self-renewal and tumorigenesis targeting the arginine methylome," Nature Communications, Nature, vol. 14(1), pages 1-21, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36410-6
    DOI: 10.1038/s41467-023-36410-6
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    References listed on IDEAS

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    2. Liang Zhou & Yinghui Wang & Meijuan Zhou & Ying Zhang & Pengfei Wang & Xiaoxing Li & Jing Yang & Hongmei Wang & Zhenhua Ding, 2018. "HOXA9 inhibits HIF-1α-mediated glycolysis through interacting with CRIP2 to repress cutaneous squamous cell carcinoma development," Nature Communications, Nature, vol. 9(1), pages 1-17, December.
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