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Next generation genetically encoded fluorescent sensors for serotonin

Author

Listed:
  • Martin Kubitschke

    (University of Bremen)

  • Monika Müller

    (German Center for Neurodegenerative Diseases (DZNE))

  • Lutz Wallhorn

    (University of Bremen)

  • Mauro Pulin

    (Center for Molecular Neurobiology (ZMNH), UKE)

  • Manuel Mittag

    (German Center for Neurodegenerative Diseases (DZNE))

  • Stefan Pollok

    (Ruhr University Bochum)

  • Tim Ziebarth

    (Ruhr University Bochum)

  • Svenja Bremshey

    (University of Bremen)

  • Jill Gerdey

    (University of Bremen)

  • Kristin Carolin Claussen

    (University of Bremen)

  • Kim Renken

    (University of Bremen)

  • Juliana Groß

    (University of Bremen)

  • Pascal Gneiße

    (University of Bremen)

  • Niklas Meyer

    (University of Bremen)

  • J. Simon Wiegert

    (Center for Molecular Neurobiology (ZMNH), UKE
    University of Heidelberg, Mannheim)

  • Andreas Reiner

    (Ruhr University Bochum)

  • Martin Fuhrmann

    (German Center for Neurodegenerative Diseases (DZNE))

  • Olivia Andrea Masseck

    (University of Bremen)

Abstract

We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging.

Suggested Citation

  • Martin Kubitschke & Monika Müller & Lutz Wallhorn & Mauro Pulin & Manuel Mittag & Stefan Pollok & Tim Ziebarth & Svenja Bremshey & Jill Gerdey & Kristin Carolin Claussen & Kim Renken & Juliana Groß & , 2022. "Next generation genetically encoded fluorescent sensors for serotonin," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35200-w
    DOI: 10.1038/s41467-022-35200-w
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    References listed on IDEAS

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    1. Melissa R. Warden & Aslihan Selimbeyoglu & Julie J. Mirzabekov & Maisie Lo & Kimberly R. Thompson & Sung-Yon Kim & Avishek Adhikari & Kay M. Tye & Loren M. Frank & Karl Deisseroth, 2012. "A prefrontal cortex–brainstem neuronal projection that controls response to behavioural challenge," Nature, Nature, vol. 492(7429), pages 428-432, December.
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