IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-33858-w.html
   My bibliography  Save this article

Structural insight into the ligand binding mechanism of aryl hydrocarbon receptor

Author

Listed:
  • Shuyan Dai

    (Central South University)

  • Lingzhi Qu

    (Central South University)

  • Jun Li

    (The First Affiliated Hospital of University of South China)

  • Ye Zhang

    (Central South University)

  • Longying Jiang

    (Central South University)

  • Hudie Wei

    (Central South University)

  • Ming Guo

    (Central South University)

  • Xiaojuan Chen

    (Central South University)

  • Yongheng Chen

    (Central South University)

Abstract

The aryl hydrocarbon receptor (AHR), a member of the basic helix–loop–helix (bHLH) Per–Arnt–Sim (PAS) family of transcription factors, plays important roles in regulating xenobiotic metabolism, cellular differentiation, stem cell maintenance, as well as immunity. More recently, AHR has gained significant interest as a drug target for the development of novel cancer immunotherapy drugs. Detailed understanding of AHR-ligand binding has been hampered for decades by the lack of a three-dimensional structure of the AHR PAS-B domain. Here, we present multiple crystal structures of the Drosophila AHR PAS-B domain, including its apo, ligand-bound, and AHR nuclear translocator (ARNT) PAS-B-bound forms. Together with biochemical and cellular assays, our data reveal structural features of the AHR PAS-B domain, provide insights into the mechanism of AHR ligand binding, and provide the structural basis for the future development of AHR-targeted therapeutics.

Suggested Citation

  • Shuyan Dai & Lingzhi Qu & Jun Li & Ye Zhang & Longying Jiang & Hudie Wei & Ming Guo & Xiaojuan Chen & Yongheng Chen, 2022. "Structural insight into the ligand binding mechanism of aryl hydrocarbon receptor," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33858-w
    DOI: 10.1038/s41467-022-33858-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-33858-w
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-022-33858-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Wenfang Chen & Haley Hill & Alana Christie & Min Soo Kim & Eboni Holloman & Andrea Pavia-Jimenez & Farrah Homayoun & Yuanqing Ma & Nirav Patel & Paul Yell & Guiyang Hao & Qurratulain Yousuf & Allison , 2016. "Targeting renal cell carcinoma with a HIF-2 antagonist," Nature, Nature, vol. 539(7627), pages 112-117, November.
    2. Dalei Wu & Nalini Potluri & Jingping Lu & Youngchang Kim & Fraydoon Rastinejad, 2015. "Structural integration in hypoxia-inducible factors," Nature, Nature, vol. 524(7565), pages 303-308, August.
    3. Luis Felipe Campesato & Sadna Budhu & Jeremy Tchaicha & Chien-Huan Weng & Mathieu Gigoux & Ivan Jose Cohen & David Redmond & Levi Mangarin & Stephane Pourpe & Cailian Liu & Roberta Zappasodi & Dmitriy, 2020. "Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    4. Sébastien Corre & Nina Tardif & Nicolas Mouchet & Héloïse M. Leclair & Lise Boussemart & Arthur Gautron & Laura Bachelot & Anthony Perrot & Anatoly Soshilov & Aljosja Rogiers & Florian Rambow & Erwan , 2018. "Sustained activation of the Aryl hydrocarbon Receptor transcription factor promotes resistance to BRAF-inhibitors in melanoma," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Karolína Ondrová & Iveta Zůvalová & Barbora Vyhlídalová & Kristýna Krasulová & Eva Miková & Radim Vrzal & Petr Nádvorník & Binod Nepal & Sandhya Kortagere & Martina Kopečná & David Kopečný & Marek Šeb, 2023. "Monoterpenoid aryl hydrocarbon receptor allosteric antagonists protect against ultraviolet skin damage in female mice," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Tianyu Tang & Xing Huang & Minghao Lu & Gang Zhang & Xu Han & Tingbo Liang, 2023. "Transcriptional control of pancreatic cancer immunosuppression by metabolic enzyme CD73 in a tumor-autonomous and -autocrine manner," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Enni Chen & Jiawei Wu & Jiajia Huang & Wancui Zhu & Haohui Sun & Xiaonan Wang & Dagui Lin & Xiaodi Li & Dingbo Shi & Zhiqiao Liu & Jinsheng Huang & Miao Chen & Fangyun Xie & Wuguo Deng, 2024. "FLI1 promotes IFN-γ-induced kynurenine production to impair anti-tumor immunity," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    4. Xiaotong Diao & Fei Ye & Meina Zhang & Xintong Ren & Xiaoxu Tian & Jingping Lu & Xiangnan Sun & Zeng Hou & Xiaoyu Chen & Fengwei Li & Jingjing Zhuang & Hong Ding & Chao Peng & Fraydoon Rastinejad & Ch, 2022. "Identification of oleoylethanolamide as an endogenous ligand for HIF-3α," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    5. Bo Wang & Jing Chen & Julia S. Caserto & Xi Wang & Minglin Ma, 2022. "An in situ hydrogel-mediated chemo-immunometabolic cancer therapy," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33858-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.