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Analytical and computational workflow for in-depth analysis of oxidized complex lipids in blood plasma

Author

Listed:
  • Angela Criscuolo

    (University of Leipzig
    University of Leipzig
    Thermo Fisher Scientific)

  • Palina Nepachalovich

    (Center of Membrane Biochemistry and Lipid Research, Faculty of Medicine Carl Gustav Carus of TU Dresden
    University of Leipzig
    University of Leipzig)

  • Diego Fernando Garcia-del Rio

    (University of Leipzig
    University of Leipzig)

  • Mike Lange

    (University of Leipzig
    University of Leipzig)

  • Zhixu Ni

    (Center of Membrane Biochemistry and Lipid Research, Faculty of Medicine Carl Gustav Carus of TU Dresden
    University of Leipzig
    University of Leipzig)

  • Massimo Baroni

    (Molecular Discovery, Kinetic Business Centre)

  • Gabriele Cruciani

    (University of Perugia)

  • Laura Goracci

    (University of Perugia)

  • Matthias Blüher

    (University of Leipzig
    Obesity and Vascular Research (HI-MAG) of the Helmholtz Zentrum München at the University of Leipzig and University Hospital Leipzig)

  • Maria Fedorova

    (Center of Membrane Biochemistry and Lipid Research, Faculty of Medicine Carl Gustav Carus of TU Dresden
    University of Leipzig
    University of Leipzig)

Abstract

Lipids are a structurally diverse class of biomolecules which can undergo a variety of chemical modifications. Among them, lipid (per)oxidation attracts most of the attention due to its significance in the regulation of inflammation, cell proliferation and death programs. Despite their apparent regulatory significance, the molecular repertoire of oxidized lipids remains largely elusive as accurate annotation of lipid modifications is complicated by their low abundance and often unknown, biological context-dependent structural diversity. Here, we provide a workflow based on the combination of bioinformatics and LC-MS/MS technologies to support identification and relative quantification of oxidized complex lipids in a modification type- and position-specific manner. The developed methodology is used to identify epilipidomics signatures of lean and obese individuals with and without type 2 diabetes. The characteristic signature of lipid modifications in lean individuals, dominated by the presence of modified octadecanoid acyl chains in phospho- and neutral lipids, is drastically shifted towards lipid peroxidation-driven accumulation of oxidized eicosanoids, suggesting significant alteration of endocrine signalling by oxidized lipids in metabolic disorders.

Suggested Citation

  • Angela Criscuolo & Palina Nepachalovich & Diego Fernando Garcia-del Rio & Mike Lange & Zhixu Ni & Massimo Baroni & Gabriele Cruciani & Laura Goracci & Matthias Blüher & Maria Fedorova, 2022. "Analytical and computational workflow for in-depth analysis of oxidized complex lipids in blood plasma," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33225-9
    DOI: 10.1038/s41467-022-33225-9
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    References listed on IDEAS

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    1. Filippo Veglia & Vladimir A. Tyurin & Dariush Mohammadyani & Maria Blasi & Elizabeth K. Duperret & Laxminarasimha Donthireddy & Ayumi Hashimoto & Alexandr Kapralov & Andrew Amoscato & Roberto Angelini, 2017. "Lipid bodies containing oxidatively truncated lipids block antigen cross-presentation by dendritic cells in cancer," Nature Communications, Nature, vol. 8(1), pages 1-16, December.
    2. Yuta Matsuoka & Masatomo Takahashi & Yuki Sugiura & Yoshihiro Izumi & Kazuhiro Nishiyama & Motohiro Nishida & Makoto Suematsu & Takeshi Bamba & Ken-ichi Yamada, 2021. "Structural library and visualization of endogenously oxidized phosphatidylcholines using mass spectrometry-based techniques," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
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