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Structural library and visualization of endogenously oxidized phosphatidylcholines using mass spectrometry-based techniques

Author

Listed:
  • Yuta Matsuoka

    (Kyushu University)

  • Masatomo Takahashi

    (Kyushu University)

  • Yuki Sugiura

    (Keio University School of Medicine)

  • Yoshihiro Izumi

    (Kyushu University)

  • Kazuhiro Nishiyama

    (Kyushu University)

  • Motohiro Nishida

    (Kyushu University
    National Institutes of Natural Sciences)

  • Makoto Suematsu

    (Keio University School of Medicine)

  • Takeshi Bamba

    (Kyushu University)

  • Ken-ichi Yamada

    (Kyushu University)

Abstract

Although oxidized phosphatidylcholines (oxPCs) play critical roles in numerous pathological events, the type and production sites of endogenous oxPCs remain unknown because of the lack of structural information and dedicated analytical methods. Herein, a library of 465 oxPCs is constructed using high-resolution mass spectrometry-based non-targeted analytical methods and employed to detect 70 oxPCs in mice with acetaminophen-induced acute liver failure. We show that doubly oxygenated polyunsaturated fatty acid (PUFA)-PCs (PC PUFA;O2), containing epoxy and hydroxide groups, are generated in the early phase of liver injury. Hybridization with in-vivo 18O labeling and matrix-assisted laser desorption/ionization-tandem MS imaging reveals that PC PUFA;O2 are accumulated in cytochrome P450 2E1-expressing and glutathione-depleted hepatocytes, which are the major sites of liver injury. The developed library and visualization methodology should facilitate the characterization of specific lipid peroxidation events and enhance our understanding of their physiological and pathological significance in lipid peroxidation-related diseases.

Suggested Citation

  • Yuta Matsuoka & Masatomo Takahashi & Yuki Sugiura & Yoshihiro Izumi & Kazuhiro Nishiyama & Motohiro Nishida & Makoto Suematsu & Takeshi Bamba & Ken-ichi Yamada, 2021. "Structural library and visualization of endogenously oxidized phosphatidylcholines using mass spectrometry-based techniques," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26633-w
    DOI: 10.1038/s41467-021-26633-w
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    Cited by:

    1. Angela Criscuolo & Palina Nepachalovich & Diego Fernando Garcia-del Rio & Mike Lange & Zhixu Ni & Massimo Baroni & Gabriele Cruciani & Laura Goracci & Matthias Blüher & Maria Fedorova, 2022. "Analytical and computational workflow for in-depth analysis of oxidized complex lipids in blood plasma," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Naoya Yamada & Tadayoshi Karasawa & Junya Ito & Daisuke Yamamuro & Kazushi Morimoto & Toshitaka Nakamura & Takanori Komada & Chintogtokh Baatarjav & Yuma Saimoto & Yuka Jinnouchi & Kazuhisa Watanabe &, 2024. "Inhibition of 7-dehydrocholesterol reductase prevents hepatic ferroptosis under an active state of sterol synthesis," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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