IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-32414-w.html
   My bibliography  Save this article

Biocatalytic routes to stereo-divergent iridoids

Author

Listed:
  • Néstor J. Hernández Lozada

    (Max-Planck Institute for Chemical Ecology, Department of Natural Product Biosynthesis)

  • Benke Hong

    (Max-Planck Institute for Chemical Ecology, Department of Natural Product Biosynthesis)

  • Joshua C. Wood

    (Center for Applied Genetic Technologies, University of Georgia)

  • Lorenzo Caputi

    (Max-Planck Institute for Chemical Ecology, Department of Natural Product Biosynthesis)

  • Jérôme Basquin

    (Max-Planck Institute for Biochemistry, Department of Structural Cell Biology)

  • Ling Chuang

    (Max-Planck Institute for Chemical Ecology, Department of Natural Product Biosynthesis)

  • Maritta Kunert

    (Max-Planck Institute for Chemical Ecology, Department of Natural Product Biosynthesis)

  • Carlos E. Rodríguez López

    (Max-Planck Institute for Chemical Ecology, Department of Natural Product Biosynthesis)

  • Chloe Langley

    (Max-Planck Institute for Chemical Ecology, Department of Natural Product Biosynthesis)

  • Dongyan Zhao

    (Center for Applied Genetic Technologies, University of Georgia)

  • C. Robin Buell

    (Center for Applied Genetic Technologies, University of Georgia)

  • Benjamin R. Lichman

    (University of York, Department of Biology, Centre for Agricultural Products)

  • Sarah E. O’Connor

    (Max-Planck Institute for Chemical Ecology, Department of Natural Product Biosynthesis)

Abstract

Thousands of natural products are derived from the fused cyclopentane-pyran molecular scaffold nepetalactol. These natural products are used in an enormous range of applications that span the agricultural and medical industries. For example, nepetalactone, the oxidized derivative of nepetalactol, is known for its cat attractant properties as well as potential as an insect repellent. Most of these naturally occurring nepetalactol-derived compounds arise from only two out of the eight possible stereoisomers, 7S-cis-trans and 7R-cis-cis nepetalactols. Here we use a combination of naturally occurring and engineered enzymes to produce seven of the eight possible nepetalactol or nepetalactone stereoisomers. These enzymes open the possibilities for biocatalytic production of a broader range of iridoids, providing a versatile system for the diversification of this important natural product scaffold.

Suggested Citation

  • Néstor J. Hernández Lozada & Benke Hong & Joshua C. Wood & Lorenzo Caputi & Jérôme Basquin & Ling Chuang & Maritta Kunert & Carlos E. Rodríguez López & Chloe Langley & Dongyan Zhao & C. Robin Buell & , 2022. "Biocatalytic routes to stereo-divergent iridoids," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32414-w
    DOI: 10.1038/s41467-022-32414-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-32414-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-32414-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Fernando Geu-Flores & Nathaniel H. Sherden & Vincent Courdavault & Vincent Burlat & Weslee S. Glenn & Cen Wu & Ezekiel Nims & Yuehua Cui & Sarah E. O’Connor, 2012. "An alternative route to cyclic terpenes by reductive cyclization in iridoid biosynthesis," Nature, Nature, vol. 492(7427), pages 138-142, December.
    2. Karel Miettinen & Lemeng Dong & Nicolas Navrot & Thomas Schneider & Vincent Burlat & Jacob Pollier & Lotte Woittiez & Sander van der Krol & Raphaël Lugan & Tina Ilc & Robert Verpoorte & Kirsi-Marja Ok, 2014. "The seco-iridoid pathway from Catharanthus roseus," Nature Communications, Nature, vol. 5(1), pages 1-12, May.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Zhong Li & Lilan Zhang & Kangwei Xu & Yuanyuan Jiang & Jieke Du & Xingwang Zhang & Ling-Hong Meng & Qile Wu & Lei Du & Xiaoju Li & Yuechan Hu & Zhenzhen Xie & Xukai Jiang & Ya-Jie Tang & Ruibo Wu & Re, 2023. "Molecular insights into the catalytic promiscuity of a bacterial diterpene synthase," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32414-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.