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Photonic crystal enhanced fluorescence emission and blinking suppression for single quantum dot digital resolution biosensing

Author

Listed:
  • Yanyu Xiong

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana−Champaign)

  • Qinglan Huang

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana−Champaign)

  • Taylor D. Canady

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana−Champaign)

  • Priyash Barya

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana−Champaign)

  • Shengyan Liu

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana−Champaign)

  • Opeyemi H. Arogundade

    (University of Illinois at Urbana-Champaign)

  • Caitlin M. Race

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana−Champaign)

  • Congnyu Che

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana-Champaign)

  • Xiaojing Wang

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana−Champaign)

  • Lifeng Zhou

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana−Champaign)

  • Xing Wang

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana−Champaign
    University of Illinois at Urbana-Champaign)

  • Manish Kohli

    (Huntsman Cancer Institute)

  • Andrew M. Smith

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana-Champaign
    Carle Illinois College of Medicine
    University of Illinois at Urbana-Champaign)

  • Brian T. Cunningham

    (University of Illinois at Urbana−Champaign
    University of Illinois at Urbana−Champaign
    University of Illinois at Urbana−Champaign
    University of Illinois at Urbana-Champaign)

Abstract

While nanoscale quantum emitters are effective tags for measuring biomolecular interactions, their utilities for applications that demand single-unit observations are limited by the requirements for large numerical aperture (NA) objectives, fluorescence intermittency, and poor photon collection efficiency resulted from omnidirectional emission. Here, we report a nearly 3000-fold signal enhancement achieved through multiplicative effects of enhanced excitation, highly directional extraction, quantum efficiency improvement, and blinking suppression through a photonic crystal (PC) surface. The approach achieves single quantum dot (QD) sensitivity with high signal-to-noise ratio, even when using a low-NA lens and an inexpensive optical setup. The blinking suppression capability of the PC improves the QDs on-time from 15% to 85% ameliorating signal intermittency. We developed an assay for cancer-associated miRNA biomarkers with single-molecule resolution, single-base mutation selectivity, and 10-attomolar detection limit. Additionally, we observed differential surface motion trajectories of QDs when their surface attachment stringency is altered by changing a single base in a cancer-specific miRNA sequence.

Suggested Citation

  • Yanyu Xiong & Qinglan Huang & Taylor D. Canady & Priyash Barya & Shengyan Liu & Opeyemi H. Arogundade & Caitlin M. Race & Congnyu Che & Xiaojing Wang & Lifeng Zhou & Xing Wang & Manish Kohli & Andrew , 2022. "Photonic crystal enhanced fluorescence emission and blinking suppression for single quantum dot digital resolution biosensing," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32387-w
    DOI: 10.1038/s41467-022-32387-w
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    References listed on IDEAS

    as
    1. Gaolian Xu & Julien Reboud & Yunfei Guo & Hao Yang & Hongchen Gu & Chunhai Fan & Xiaohua Qian & Jonathan M. Cooper, 2022. "Programmable design of isothermal nucleic acid diagnostic assays through abstraction-based models," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    2. Sung Jun Lim & Mohammad U. Zahid & Phuong Le & Liang Ma & David Entenberg & Allison S. Harney & John Condeelis & Andrew M. Smith, 2015. "Brightness-equalized quantum dots," Nature Communications, Nature, vol. 6(1), pages 1-10, November.
    3. Phuong Le & Sung Jun Lim & Brian C. Baculis & Hee Jung Chung & Kristopher A. Kilian & Andrew M. Smith, 2019. "Counting growth factors in single cells with infrared quantum dots to measure discrete stimulation distributions," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    4. Shenglin Cai & Thomas Pataillot-Meakin & Akifumi Shibakawa & Ren Ren & Charlotte L. Bevan & Sylvain Ladame & Aleksandar P. Ivanov & Joshua B. Edel, 2021. "Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
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