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Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum

Author

Listed:
  • Shenglin Cai

    (Molecular Science Research Hub)

  • Thomas Pataillot-Meakin

    (Molecular Science Research Hub
    Sir Michael Uren Hub
    Hammersmith Hospital)

  • Akifumi Shibakawa

    (Hammersmith Hospital)

  • Ren Ren

    (Molecular Science Research Hub)

  • Charlotte L. Bevan

    (Hammersmith Hospital)

  • Sylvain Ladame

    (Sir Michael Uren Hub)

  • Aleksandar P. Ivanov

    (Molecular Science Research Hub)

  • Joshua B. Edel

    (Molecular Science Research Hub)

Abstract

MicroRNAs (miRNAs) play essential roles in post-transcriptional gene expression and are also found freely circulating in bodily fluids such as blood. Dysregulated miRNA signatures have been associated with many diseases including cancer, and miRNA profiling from liquid biopsies offers a promising strategy for cancer diagnosis, prognosis and monitoring. Here, we develop size-encoded molecular probes that can be used for simultaneous electro-optical nanopore sensing of miRNAs, allowing for ultrasensitive, sequence-specific and multiplexed detection directly in unprocessed human serum, in sample volumes as small as 0.1 μl. We show that this approach allows for femtomolar sensitivity and single-base mismatch selectivity. We demonstrate the ability to simultaneously monitor miRNAs (miR-141-3p and miR-375-3p) from prostate cancer patients with active disease and in remission. This technology can pave the way for next generation of minimally invasive diagnostic and companion diagnostic tests for cancer.

Suggested Citation

  • Shenglin Cai & Thomas Pataillot-Meakin & Akifumi Shibakawa & Ren Ren & Charlotte L. Bevan & Sylvain Ladame & Aleksandar P. Ivanov & Joshua B. Edel, 2021. "Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23497-y
    DOI: 10.1038/s41467-021-23497-y
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    Cited by:

    1. Yanyu Xiong & Qinglan Huang & Taylor D. Canady & Priyash Barya & Shengyan Liu & Opeyemi H. Arogundade & Caitlin M. Race & Congnyu Che & Xiaojing Wang & Lifeng Zhou & Xing Wang & Manish Kohli & Andrew , 2022. "Photonic crystal enhanced fluorescence emission and blinking suppression for single quantum dot digital resolution biosensing," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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