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Programmable CRISPR-Cas9 microneedle patch for long-term capture and real-time monitoring of universal cell-free DNA

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  • Bin Yang

    (Fudan University)

  • Jilie Kong

    (Fudan University)

  • Xueen Fang

    (Fudan University)

Abstract

Recent advances in biointerfaces have led to the development of wearable devices that can provide insights into personal health. As wearable modules, microneedles can extract analytes of interest from interstitial fluid in a minimally invasive fashion. However, some microneedles are limited by their ability to perform highly effective extraction and real-time monitoring for macromolecule biomarkers simultaneously. Here we show the synergetic effect of CRISPR-activated graphene biointerfaces, and report an on-line wearable microneedle patch for extraction and in vivo long-term monitoring of universal cell-free DNA. In this study, this wearable system enables real-time monitoring of Epstein-Barr virus, sepsis, and kidney transplantation cell-free DNA, with anti-interference ability of 60% fetal bovine serum, and has satisfactory stable sensitivity for 10 days in vivo. The experimental results of immunodeficient mouse models shows the feasibility and practicability of this proposed method. This wearable patch holds great promise for long-term in vivo monitoring of cell-free DNA and could potentially be used for early disease screening and prognosis.

Suggested Citation

  • Bin Yang & Jilie Kong & Xueen Fang, 2022. "Programmable CRISPR-Cas9 microneedle patch for long-term capture and real-time monitoring of universal cell-free DNA," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31740-3
    DOI: 10.1038/s41467-022-31740-3
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    1. Lei Dai & Xueliang Cui & Xin Zhang & Lin Cheng & Yi Liu & Yang Yang & Ping Fan & Qingnan Wang & Yi Lin & Junfeng Zhang & Chunlei Li & Ying Mao & Qin Wang & Xiaolan Su & Shuang Zhang & Yong Peng & Hans, 2016. "RETRACTED ARTICLE: SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin," Nature Communications, Nature, vol. 7(1), pages 1-15, November.
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    Cited by:

    1. Bin Yang & Haonan Wang & Jilie Kong & Xueen Fang, 2024. "Long-term monitoring of ultratrace nucleic acids using tetrahedral nanostructure-based NgAgo on wearable microneedles," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Bin Zheng & Qiuya Li & Laiping Fang & Xiaolu Cai & Yan Liu & Yanhong Duo & Bowen Li & Zhengyu Wu & Boxi Shen & Yang Bai & Shi-Xiang Cheng & Xingcai Zhang, 2024. "Microorganism microneedle micro-engine depth drug delivery," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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