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Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses

Author

Listed:
  • Yi-Wen Lim

    (ETH Zürich)

  • Ben N. Mansfeld

    (Donald Danforth Plant Science Center)

  • Pascal Schläpfer

    (ETH Zürich)

  • Kerrigan B. Gilbert

    (Donald Danforth Plant Science Center)

  • Narayanan N. Narayanan

    (Donald Danforth Plant Science Center)

  • Weihong Qi

    (ETH Zurich and University of Zurich)

  • Qi Wang

    (Donald Danforth Plant Science Center)

  • Zhenhui Zhong

    (University of California Los Angeles)

  • Adam Boyher

    (Donald Danforth Plant Science Center)

  • Jackson Gehan

    (Donald Danforth Plant Science Center)

  • Getu Beyene

    (Donald Danforth Plant Science Center)

  • Zuh-Jyh Daniel Lin

    (Donald Danforth Plant Science Center)

  • Williams Esuma

    (National Crops Resources Research Institute)

  • Suhua Feng

    (University of California Los Angeles)

  • Christelle Chanez

    (ETH Zürich)

  • Nadine Eggenberger

    (ETH Zürich)

  • Gerald Adiga

    (National Crops Resources Research Institute)

  • Titus Alicai

    (National Crops Resources Research Institute)

  • Steven E. Jacobsen

    (University of California Los Angeles
    Howard Hughes Medical Institute University of California Los Angeles)

  • Nigel J. Taylor

    (Donald Danforth Plant Science Center)

  • Wilhelm Gruissem

    (ETH Zürich
    National Chung Hsing University)

  • Rebecca S. Bart

    (Donald Danforth Plant Science Center)

Abstract

Cassava mosaic disease (CMD) suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to cassava mosaic geminiviruses. It has been reported that CMD2-type landraces lose resistance after regeneration through de novo morphogenesis. As full genome bisulfite sequencing failed to uncover an epigenetic mechanism for this loss of resistance, whole genome sequencing and genetic variant analysis was performed and the CMD2 locus was fine-mapped to a 190 kilobase interval. Collectively, these data indicate that CMD2-type resistance is caused by a nonsynonymous, single nucleotide polymorphism in DNA polymerase δ subunit 1 (MePOLD1) located within this region. Virus-induced gene silencing of MePOLD1 in a CMD-susceptible cassava variety produced a recovery phenotype typical of CMD2-type resistance. Analysis of other CMD2-type cassava varieties identified additional candidate resistance alleles within MePOLD1. Genetic variation of MePOLD1, therefore, could represent an important genetic resource for resistance breeding and/or genome editing, and elucidating mechanisms of resistance to geminiviruses.

Suggested Citation

  • Yi-Wen Lim & Ben N. Mansfeld & Pascal Schläpfer & Kerrigan B. Gilbert & Narayanan N. Narayanan & Weihong Qi & Qi Wang & Zhenhui Zhong & Adam Boyher & Jackson Gehan & Getu Beyene & Zuh-Jyh Daniel Lin &, 2022. "Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31414-0
    DOI: 10.1038/s41467-022-31414-0
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    References listed on IDEAS

    as
    1. Mengshi Wu & Hua Wei & Huang Tan & Shaojun Pan & Qi Liu & Eduardo R. Bejarano & Rosa Lozano-Durán, 2021. "Plant DNA polymerases α and δ mediate replication of geminiviruses," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    2. Meilina Ong-Abdullah & Jared M. Ordway & Nan Jiang & Siew-Eng Ooi & Sau-Yee Kok & Norashikin Sarpan & Nuraziyan Azimi & Ahmad Tarmizi Hashim & Zamzuri Ishak & Samsul Kamal Rosli & Fadila Ahmad Malike , 2015. "Loss of Karma transposon methylation underlies the mantled somaclonal variant of oil palm," Nature, Nature, vol. 525(7570), pages 533-537, September.
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