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Plant DNA polymerases α and δ mediate replication of geminiviruses

Author

Listed:
  • Mengshi Wu

    (Chinese Academy of Sciences
    Tongji University
    University of the Chinese Academy of Sciences)

  • Hua Wei

    (Chinese Academy of Sciences
    University of the Chinese Academy of Sciences)

  • Huang Tan

    (Chinese Academy of Sciences
    University of the Chinese Academy of Sciences)

  • Shaojun Pan

    (Chinese Academy of Sciences
    University of the Chinese Academy of Sciences)

  • Qi Liu

    (Tongji University)

  • Eduardo R. Bejarano

    (Universidad de Málaga, Campus de Teatinos s/n)

  • Rosa Lozano-Durán

    (Chinese Academy of Sciences
    Eberhard Karls University)

Abstract

Geminiviruses are causal agents of devastating diseases in crops. Geminiviruses have circular single-stranded (ss) DNA genomes that are replicated in the nucleus of the infected plant cell through double-stranded (ds) DNA intermediates by the plant DNA replication machinery. Which host DNA polymerase mediates geminiviral multiplication, however, has so far remained elusive. Here, we show that subunits of the nuclear replicative DNA polymerases α and δ physically interact with the geminivirus-encoded replication enhancer protein, C3, and that these polymerases are required for viral replication. Our results suggest that, while DNA polymerase α is essential to generate the viral dsDNA intermediate, DNA polymerase δ mediates the synthesis of new copies of the geminiviral ssDNA genome, and that the virus-encoded C3 may act selectively, recruiting DNA polymerase δ over ε to favour productive replication.

Suggested Citation

  • Mengshi Wu & Hua Wei & Huang Tan & Shaojun Pan & Qi Liu & Eduardo R. Bejarano & Rosa Lozano-Durán, 2021. "Plant DNA polymerases α and δ mediate replication of geminiviruses," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23013-2
    DOI: 10.1038/s41467-021-23013-2
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    Cited by:

    1. Yi-Wen Lim & Ben N. Mansfeld & Pascal Schläpfer & Kerrigan B. Gilbert & Narayanan N. Narayanan & Weihong Qi & Qi Wang & Zhenhui Zhong & Adam Boyher & Jackson Gehan & Getu Beyene & Zuh-Jyh Daniel Lin &, 2022. "Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

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