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Insights into the mechanism of action of the arbitrium communication system in SPbeta phages

Author

Listed:
  • Francisca Gallego del Sol

    (CSIC and CIBER de Enfermedades Raras (CIBERER))

  • Nuria Quiles-Puchalt

    (Imperial College London)

  • Aisling Brady

    (Imperial College London
    University of Glasgow)

  • José R. Penadés

    (Imperial College London)

  • Alberto Marina

    (CSIC and CIBER de Enfermedades Raras (CIBERER))

Abstract

The arbitrium system is employed by phages of the SPbeta family to communicate with their progeny during infection to decide either to follow the lytic or the lysogenic cycle. The system is controlled by a peptide, AimP, that binds to the regulator AimR, inhibiting its DNA-binding activity and expression of aimX. Although the structure of AimR has been elucidated for phages SPβ and phi3T, there is still controversy regarding the molecular mechanism of AimR function, with two different proposed models for SPβ. In this study, we deepen our understanding of the system by solving the structure of an additional AimR that shows chimerical characteristics with the SPβ receptor. The crystal structures of this AimR (apo, AimP-bound and DNA-bound) together with in vitro and in vivo analyses confirm a mechanism of action by AimP-induced conformational restriction, shedding light on peptide specificity and cross regulation with relevant biological implications.

Suggested Citation

  • Francisca Gallego del Sol & Nuria Quiles-Puchalt & Aisling Brady & José R. Penadés & Alberto Marina, 2022. "Insights into the mechanism of action of the arbitrium communication system in SPbeta phages," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31144-3
    DOI: 10.1038/s41467-022-31144-3
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    References listed on IDEAS

    as
    1. J. Rafael Ciges-Tomas & Christian Alite & Suzanne Humphrey & J. Donderis & Janine Bowring & Xavier Salvatella & José R. Penadés & Alberto Marina, 2019. "The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
    2. Elie Dolgin, 2019. "The secret social lives of viruses," Nature, Nature, vol. 570(7761), pages 290-292, June.
    3. Zohar Erez & Ida Steinberger-Levy & Maya Shamir & Shany Doron & Avigail Stokar-Avihail & Yoav Peleg & Sarah Melamed & Azita Leavitt & Alon Savidor & Shira Albeck & Gil Amitai & Rotem Sorek, 2017. "Communication between viruses guides lysis–lysogeny decisions," Nature, Nature, vol. 541(7638), pages 488-493, January.
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