IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-31144-3.html
   My bibliography  Save this article

Insights into the mechanism of action of the arbitrium communication system in SPbeta phages

Author

Listed:
  • Francisca Gallego del Sol

    (CSIC and CIBER de Enfermedades Raras (CIBERER))

  • Nuria Quiles-Puchalt

    (Imperial College London)

  • Aisling Brady

    (Imperial College London
    University of Glasgow)

  • José R. Penadés

    (Imperial College London)

  • Alberto Marina

    (CSIC and CIBER de Enfermedades Raras (CIBERER))

Abstract

The arbitrium system is employed by phages of the SPbeta family to communicate with their progeny during infection to decide either to follow the lytic or the lysogenic cycle. The system is controlled by a peptide, AimP, that binds to the regulator AimR, inhibiting its DNA-binding activity and expression of aimX. Although the structure of AimR has been elucidated for phages SPβ and phi3T, there is still controversy regarding the molecular mechanism of AimR function, with two different proposed models for SPβ. In this study, we deepen our understanding of the system by solving the structure of an additional AimR that shows chimerical characteristics with the SPβ receptor. The crystal structures of this AimR (apo, AimP-bound and DNA-bound) together with in vitro and in vivo analyses confirm a mechanism of action by AimP-induced conformational restriction, shedding light on peptide specificity and cross regulation with relevant biological implications.

Suggested Citation

  • Francisca Gallego del Sol & Nuria Quiles-Puchalt & Aisling Brady & José R. Penadés & Alberto Marina, 2022. "Insights into the mechanism of action of the arbitrium communication system in SPbeta phages," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31144-3
    DOI: 10.1038/s41467-022-31144-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-31144-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-31144-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. J. Rafael Ciges-Tomas & Christian Alite & Suzanne Humphrey & J. Donderis & Janine Bowring & Xavier Salvatella & José R. Penadés & Alberto Marina, 2019. "The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
    2. Elie Dolgin, 2019. "The secret social lives of viruses," Nature, Nature, vol. 570(7761), pages 290-292, June.
    3. Zohar Erez & Ida Steinberger-Levy & Maya Shamir & Shany Doron & Avigail Stokar-Avihail & Yoav Peleg & Sarah Melamed & Azita Leavitt & Alon Savidor & Shira Albeck & Gil Amitai & Rotem Sorek, 2017. "Communication between viruses guides lysis–lysogeny decisions," Nature, Nature, vol. 541(7638), pages 488-493, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yuncong Geng & Thu Vu Phuc Nguyen & Ehsan Homaee & Ido Golding, 2024. "Using bacterial population dynamics to count phages and their lysogens," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    2. Asher Leeks & Stuart A. West & Melanie Ghoul, 2021. "The evolution of cheating in viruses," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    3. Koen Wortelboer & Patrick A. Jonge & Torsten P. M. Scheithauer & Ilias Attaye & E. Marleen Kemper & Max Nieuwdorp & Hilde Herrema, 2023. "Phage-microbe dynamics after sterile faecal filtrate transplantation in individuals with metabolic syndrome: a double-blind, randomised, placebo-controlled clinical trial assessing efficacy and safety," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    4. Hildebrandt, Mireille, 2020. "Smart technologies," Internet Policy Review: Journal on Internet Regulation, Alexander von Humboldt Institute for Internet and Society (HIIG), Berlin, vol. 9(4), pages 1-16.
    5. Shengbo Wu & Jie Feng & Chunjiang Liu & Hao Wu & Zekai Qiu & Jianjun Ge & Shuyang Sun & Xia Hong & Yukun Li & Xiaona Wang & Aidong Yang & Fei Guo & Jianjun Qiao, 2022. "Machine learning aided construction of the quorum sensing communication network for human gut microbiota," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    6. Angela Tung & Megan M. Sperry & Wesley Clawson & Ananya Pavuluri & Sydney Bulatao & Michelle Yue & Ramses Martinez Flores & Vaibhav P. Pai & Patrick McMillen & Franz Kuchling & Michael Levin, 2024. "Embryos assist morphogenesis of others through calcium and ATP signaling mechanisms in collective teratogen resistance," Nature Communications, Nature, vol. 15(1), pages 1-22, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31144-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.