IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-30940-1.html
   My bibliography  Save this article

SMYD5 catalyzes histone H3 lysine 36 trimethylation at promoters

Author

Listed:
  • Yanjun Zhang

    (Zhejiang University)

  • Yuan Fang

    (Zhejiang University)

  • Yin Tang

    (Zhejiang University)

  • Shixun Han

    (Zhejiang University)

  • Junqi Jia

    (Zhejiang University)

  • Xinyi Wan

    (Zhejiang University)

  • Jiaqi Chen

    (Zhejiang University School of Medicine)

  • Ying Yuan

    (Zhejiang University School of Medicine)

  • Bin Zhao

    (Zhejiang University)

  • Dong Fang

    (Zhejiang University
    Zhejiang University School of Medicine)

Abstract

Histone marks, carriers of epigenetic information, regulate gene expression. In mammalian cells, H3K36me3 is mainly catalyzed by SETD2 at gene body regions. Here, we find that in addition to gene body regions, H3K36me3 is enriched at promoters in primary cells. Through screening, we identify SMYD5, which is recruited to chromatin by RNA polymerase II, as a methyltransferase catalyzing H3K36me3 at promoters. The enzymatic activity of SMYD5 is dependent on its C-terminal glutamic acid-rich domain. Overexpression of full-length Smyd5, but not the C-terminal domain-truncated Smyd5, restores H3K36me3 at promoters in Smyd5 knockout cells. Furthermore, elevated Smyd5 expression contributes to tumorigenesis in liver hepatocellular carcinoma. Together, our findings identify SMYD5 as the H3K36me3 methyltransferase at promoters that regulates gene expression, providing insights into the localization and function of H3K36me3.

Suggested Citation

  • Yanjun Zhang & Yuan Fang & Yin Tang & Shixun Han & Junqi Jia & Xinyi Wan & Jiaqi Chen & Ying Yuan & Bin Zhao & Dong Fang, 2022. "SMYD5 catalyzes histone H3 lysine 36 trimethylation at promoters," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30940-1
    DOI: 10.1038/s41467-022-30940-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-30940-1
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-022-30940-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Huilin Huang & Hengyou Weng & Keren Zhou & Tong Wu & Boxuan Simen Zhao & Mingli Sun & Zhenhua Chen & Xiaolan Deng & Gang Xiao & Franziska Auer & Lars Klemm & Huizhe Wu & Zhixiang Zuo & Xi Qin & Yunzhu, 2019. "Histone H3 trimethylation at lysine 36 guides m6A RNA modification co-transcriptionally," Nature, Nature, vol. 567(7748), pages 414-419, March.
    2. Hong Wen & Yuanyuan Li & Yuanxin Xi & Shiming Jiang & Sabrina Stratton & Danni Peng & Kaori Tanaka & Yongfeng Ren & Zheng Xia & Jun Wu & Bing Li & Michelle C. Barton & Wei Li & Haitao Li & Xiaobing Sh, 2014. "ZMYND11 links histone H3.3K36me3 to transcription elongation and tumour suppression," Nature, Nature, vol. 508(7495), pages 263-268, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Chirag Nepal & Jesper B. Andersen, 2023. "Alternative promoters in CpG depleted regions are prevalently associated with epigenetic misregulation of liver cancer transcriptomes," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Xin Yang & Robinson Triboulet & Qi Liu & Erdem Sendinc & Richard I. Gregory, 2022. "Exon junction complex shapes the m6A epitranscriptome," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Yongli Shan & Yanqi Zhang & Yanxing Wei & Cong Zhang & Huaisong Lin & Jiangping He & Junwei Wang & Wenjing Guo & Heying Li & Qianyu Chen & Tiancheng Zhou & Qi Xing & Yancai Liu & Jiekai Chen & Guangji, 2024. "METTL3/METTL14 maintain human nucleoli integrity by mediating SUV39H1/H2 degradation," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    4. Yuanpei Li & Xiaoniu He & Xiao Lu & Zhicheng Gong & Qing Li & Lei Zhang & Ronghui Yang & Chengyi Wu & Jialiang Huang & Jiancheng Ding & Yaohui He & Wen Liu & Ceshi Chen & Bin Cao & Dawang Zhou & Yufen, 2022. "METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions," Nature Communications, Nature, vol. 13(1), pages 1-23, December.
    5. Lunying Wu & Xiaohui Jing & Baolan Zhang & Shoujun Chen & Ran Xu & Penggen Duan & Danni Zou & Shengjian Huang & Tingbo Zhou & Chengcai An & Yuehua Luo & Yunhai Li, 2022. "A natural allele of OsMS1 responds to temperature changes and confers thermosensitive genic male sterility," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    6. Yanfen Zheng & Xingyang Li & Shuang Deng & Hongzhe Zhao & Ying Ye & Shaoping Zhang & Xudong Huang & Ruihong Bai & Lisha Zhuang & Quanbo Zhou & Mei Li & Jiachun Su & Rui Li & Xiaoqiong Bao & Lingxing Z, 2023. "CSTF2 mediated mRNA N6-methyladenosine modification drives pancreatic ductal adenocarcinoma m6A subtypes," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30940-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.