IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-30061-9.html
   My bibliography  Save this article

Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics

Author

Listed:
  • Kaori Kanemaru

    (Tokyo University of Science)

  • Makoto Shimozawa

    (Tokyo University of Pharmacy and Life Sciences)

  • Manabu Kitamata

    (Tokyo University of Pharmacy and Life Sciences)

  • Rikuto Furuishi

    (Tokyo University of Science)

  • Hinako Kayano

    (Tokyo University of Pharmacy and Life Sciences)

  • Yui Sukawa

    (Tokyo University of Pharmacy and Life Sciences)

  • Yuuki Chiba

    (Tokyo University of Pharmacy and Life Sciences)

  • Takatsugu Fukuyama

    (Tokyo University of Pharmacy and Life Sciences)

  • Junya Hasegawa

    (Tokyo Medical and Dental University
    Tokyo Medical and Dental University)

  • Hiroki Nakanishi

    (Akita University
    Lipidome Lab Co., Ltd)

  • Takuma Kishimoto

    (Hokkaido University Graduate School of Life Science)

  • Kazuya Tsujita

    (Kobe University
    Kobe University Graduate School of Medicine)

  • Kazuma Tanaka

    (Hokkaido University Graduate School of Life Science)

  • Toshiki Itoh

    (Kobe University
    Kobe University Graduate School of Medicine)

  • Junko Sasaki

    (Tokyo Medical and Dental University
    Tokyo Medical and Dental University)

  • Takehiko Sasaki

    (Tokyo Medical and Dental University
    Tokyo Medical and Dental University)

  • Kiyoko Fukami

    (Tokyo University of Pharmacy and Life Sciences)

  • Yoshikazu Nakamura

    (Tokyo University of Science)

Abstract

Epithelial cells provide cell-cell adhesion that is essential to maintain the integrity of multicellular organisms. Epithelial cell-characterizing proteins, such as epithelial junctional proteins and transcription factors are well defined. However, the role of lipids in epithelial characterization remains poorly understood. Here we show that the phospholipid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] is enriched in the plasma membrane (PM) of epithelial cells. Epithelial cells lose their characteristics upon depletion of PM PI(4,5)P2, and synthesis of PI(4,5)P2 in the PM results in the development of epithelial-like morphology in osteosarcoma cells. PM localization of PARD3 is impaired by depletion of PM PI(4,5)P2 in epithelial cells, whereas expression of the PM-targeting exocyst-docking region of PARD3 induces osteosarcoma cells to show epithelial-like morphological changes, suggesting that PI(4,5)P2 regulates epithelial characteristics by recruiting PARD3 to the PM. These results indicate that a high level of PM PI(4,5)P2 plays a crucial role in the maintenance of epithelial characteristics.

Suggested Citation

  • Kaori Kanemaru & Makoto Shimozawa & Manabu Kitamata & Rikuto Furuishi & Hinako Kayano & Yui Sukawa & Yuuki Chiba & Takatsugu Fukuyama & Junya Hasegawa & Hiroki Nakanishi & Takuma Kishimoto & Kazuya Ts, 2022. "Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30061-9
    DOI: 10.1038/s41467-022-30061-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-30061-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-30061-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Christian J Gröger & Markus Grubinger & Thomas Waldhör & Klemens Vierlinger & Wolfgang Mikulits, 2012. "Meta-Analysis of Gene Expression Signatures Defining the Epithelial to Mesenchymal Transition during Cancer Progression," PLOS ONE, Public Library of Science, vol. 7(12), pages 1-10, December.
    2. Syed Mukhtar Ahmed & Ian G. Macara, 2017. "The Par3 polarity protein is an exocyst receptor essential for mammary cell survival," Nature Communications, Nature, vol. 8(1), pages 1-15, April.
    3. Junko Sasaki & Satoshi Kofuji & Reietsu Itoh & Toshihiko Momiyama & Kiyohiko Takayama & Haruka Murakami & Shinsuke Chida & Yuko Tsuya & Shunsuke Takasuga & Satoshi Eguchi & Ken Asanuma & Yasuo Horie &, 2010. "The PtdIns(3,4)P2 phosphatase INPP4A is a suppressor of excitotoxic neuronal death," Nature, Nature, vol. 465(7297), pages 497-501, May.
    4. Kaori Kanemaru & Yoshikazu Nakamura & Kojiro Sato & Ryota Kojima & Saori Takahashi & Mami Yamaguchi & Manabu Ichinohe & Hiroshi Kiyonari & Go Shioi & Kenji Kabashima & Kyoko Nakahigashi & Masataka Asa, 2012. "Epidermal phospholipase Cδ1 regulates granulocyte counts and systemic interleukin-17 levels in mice," Nature Communications, Nature, vol. 3(1), pages 1-12, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Lining Liang & Hao Sun & Wei Zhang & Mengdan Zhang & Xiao Yang & Rui Kuang & Hui Zheng, 2016. "Meta-Analysis of EMT Datasets Reveals Different Types of EMT," PLOS ONE, Public Library of Science, vol. 11(6), pages 1-22, June.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30061-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.