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Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms

Author

Listed:
  • Mais Maree

    (University of Tsukuba)

  • Le Thuy Nguyen

    (University of Tsukuba
    Biotechnology Centre of Ho Chi Minh City, District 12)

  • Ryosuke L. Ohniwa

    (Institut Pasteur, Université Paris Cité, CNRS UMR6047, Biology of Gram-Positive Pathogens, Department of Microbiology
    University of Tsukuba)

  • Masato Higashide

    (Inc., Kamiyokoba)

  • Tarek Msadek

    (Institut Pasteur, Université Paris Cité, CNRS UMR6047, Biology of Gram-Positive Pathogens, Department of Microbiology)

  • Kazuya Morikawa

    (University of Tsukuba)

Abstract

SCCmec is a large mobile genetic element that includes the mecA gene and confers resistance to β-lactam antibiotics in methicillin-resistant Staphylococcus aureus (MRSA). There is evidence that SCCmec disseminates among staphylococci, but the transfer mechanisms are unclear. Here, we show that two-component systems mediate the upregulation of natural competence genes in S. aureus under biofilm growth conditions, and this enhances the efficiency of natural transformation. We observe SCCmec transfer via natural transformation from MRSA, and from methicillin-resistant coagulase-negative staphylococci, to methicillin-sensitive S. aureus. The process requires the SCCmec recombinase genes ccrAB, and the stability of the transferred SCCmec varies depending on SCCmec types and recipients. Our results suggest that natural transformation plays a role in the transfer of SCCmec and possibly other mobile genetic elements in S. aureus biofilms.

Suggested Citation

  • Mais Maree & Le Thuy Nguyen & Ryosuke L. Ohniwa & Masato Higashide & Tarek Msadek & Kazuya Morikawa, 2022. "Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29877-2
    DOI: 10.1038/s41467-022-29877-2
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    References listed on IDEAS

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    1. Stefany Moreno-Gámez & Robin A. Sorg & Arnau Domenech & Morten Kjos & Franz J. Weissing & G. Sander Doorn & Jan-Willem Veening, 2017. "Quorum sensing integrates environmental cues, cell density and cell history to control bacterial competence," Nature Communications, Nature, vol. 8(1), pages 1-12, December.
    2. Maite Villanueva & Begoña García & Jaione Valle & Beatriz Rapún & Igor Ruiz de los Mozos & Cristina Solano & Miguel Martí & José R. Penadés & Alejandro Toledo-Arana & Iñigo Lasa, 2018. "Sensory deprivation in Staphylococcus aureus," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
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    1. Motaher Hossain & Barbaros Aslan & Asma Hatoum-Aslan, 2024. "Tandem mobilization of anti-phage defenses alongside SCCmec elements in staphylococci," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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