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ComFC mediates transport and handling of single-stranded DNA during natural transformation

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  • Prashant P. Damke

    (Université Paris-Saclay, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob
    Université de Paris-Cité, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob)

  • Louisa Celma

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

  • Sumedha M. Kondekar

    (Université Paris-Saclay, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob
    Université de Paris-Cité, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob)

  • Anne Marie Di Guilmi

    (Université Paris-Saclay, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob
    Université de Paris-Cité, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob)

  • Stéphanie Marsin

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

  • Jordane Dépagne

    (Université Paris-Saclay, CEA, INSERM, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob
    Université de Paris Cité, CEA, INSERM, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob)

  • Xavier Veaute

    (Université Paris-Saclay, CEA, INSERM, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob
    Université de Paris Cité, CEA, INSERM, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob)

  • Pierre Legrand

    (L’Orme des Merisiers)

  • Hélène Walbott

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

  • Julien Vercruyssen

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

  • Raphaël Guérois

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

  • Sophie Quevillon-Cheruel

    (Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC))

  • J. Pablo Radicella

    (Université Paris-Saclay, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob
    Université de Paris-Cité, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob)

Abstract

The ComFC protein is essential for natural transformation, a process that plays a major role in the spread of antibiotic resistance genes and virulence factors across bacteria. However, its role remains largely unknown. Here, we show that Helicobacter pylori ComFC is involved in DNA transport through the cell membrane, and is required for the handling of the single-stranded DNA once it is delivered into the cytoplasm. The crystal structure of ComFC includes a zinc-finger motif and a putative phosphoribosyl transferase domain, both necessary for the protein’s in vivo activity. Furthermore, we show that ComFC is a membrane-associated protein with affinity for single-stranded DNA. Our results suggest that ComFC provides the link between the transport of the transforming DNA into the cytoplasm and its handling by the recombination machinery.

Suggested Citation

  • Prashant P. Damke & Louisa Celma & Sumedha M. Kondekar & Anne Marie Di Guilmi & Stéphanie Marsin & Jordane Dépagne & Xavier Veaute & Pierre Legrand & Hélène Walbott & Julien Vercruyssen & Raphaël Guér, 2022. "ComFC mediates transport and handling of single-stranded DNA during natural transformation," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29494-z
    DOI: 10.1038/s41467-022-29494-z
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    References listed on IDEAS

    as
    1. Léa Marie & Chiara Rapisarda & Violette Morales & Mathieu Bergé & Thomas Perry & Anne-Lise Soulet & Clémence Gruget & Han Remaut & Rémi Fronzes & Patrice Polard, 2017. "Bacterial RadA is a DnaB-type helicase interacting with RecA to promote bidirectional D-loop extension," Nature Communications, Nature, vol. 8(1), pages 1-14, August.
    2. Prashant P. Damke & Anne Marie Di Guilmi & Paloma Fernández Varela & Christophe Velours & Stéphanie Marsin & Xavier Veaute & Mérick Machouri & Gaurav V. Gunjal & Desirazu N. Rao & Jean-Baptiste Charbo, 2019. "Identification of the periplasmic DNA receptor for natural transformation of Helicobacter pylori," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    3. Nicolas Mirouze & Cécile Ferret & Charlène Cornilleau & Rut Carballido-López, 2018. "Antibiotic sensitivity reveals that wall teichoic acids mediate DNA binding during competence in Bacillus subtilis," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
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