IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-13352-6.html
   My bibliography  Save this article

Identification of the periplasmic DNA receptor for natural transformation of Helicobacter pylori

Author

Listed:
  • Prashant P. Damke

    (CEA, Université de Paris and Université Paris Sud
    University of Kentucky)

  • Anne Marie Di Guilmi

    (CEA, Université de Paris and Université Paris Sud)

  • Paloma Fernández Varela

    (CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay)

  • Christophe Velours

    (CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay)

  • Stéphanie Marsin

    (CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay)

  • Xavier Veaute

    (Universités Paris Diderot and Paris Sud)

  • Mérick Machouri

    (CEA, Université de Paris and Université Paris Sud)

  • Gaurav V. Gunjal

    (Indian Institute of Science)

  • Desirazu N. Rao

    (Indian Institute of Science)

  • Jean-Baptiste Charbonnier

    (CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay)

  • J. Pablo Radicella

    (CEA, Université de Paris and Université Paris Sud)

Abstract

Horizontal gene transfer through natural transformation is a major driver of antibiotic resistance spreading in many pathogenic bacterial species. In the case of Gram-negative bacteria, and in particular of Helicobacter pylori, the mechanisms underlying the handling of the incoming DNA within the periplasm are poorly understood. Here we identify the protein ComH as the periplasmic receptor for the transforming DNA during natural transformation in H. pylori. ComH is a DNA-binding protein required for the import of DNA into the periplasm. Its C-terminal domain displays strong affinity for double-stranded DNA and is sufficient for the accumulation of DNA in the periplasm, but not for DNA internalisation into the cytoplasm. The N-terminal region of the protein allows the interaction of ComH with a periplasmic domain of the inner-membrane channel ComEC, which is known to mediate the translocation of DNA into the cytoplasm. Our results indicate that ComH is involved in the import of DNA into the periplasm and its delivery to the inner membrane translocator ComEC.

Suggested Citation

  • Prashant P. Damke & Anne Marie Di Guilmi & Paloma Fernández Varela & Christophe Velours & Stéphanie Marsin & Xavier Veaute & Mérick Machouri & Gaurav V. Gunjal & Desirazu N. Rao & Jean-Baptiste Charbo, 2019. "Identification of the periplasmic DNA receptor for natural transformation of Helicobacter pylori," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13352-6
    DOI: 10.1038/s41467-019-13352-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-13352-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-13352-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Prashant P. Damke & Louisa Celma & Sumedha M. Kondekar & Anne Marie Di Guilmi & Stéphanie Marsin & Jordane Dépagne & Xavier Veaute & Pierre Legrand & Hélène Walbott & Julien Vercruyssen & Raphaël Guér, 2022. "ComFC mediates transport and handling of single-stranded DNA during natural transformation," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13352-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.