IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-29029-6.html
   My bibliography  Save this article

Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1

Author

Listed:
  • Chen Xu

    (Naval Medical University)

  • Zicheng Zhang

    (Naval Medical University
    the Fouth Medical Center of PLA General Hospital
    Naval Medical University)

  • Ning Liu

    (Naval Medical University
    923th Hospital of the Joint Logistics Support Force of PLA)

  • Li Li

    (Naval Medical University)

  • Huajian Zhong

    (Naval Medical University)

  • Ruizhe Wang

    (Naval Medical University)

  • Qianghui Shi

    (Naval Medical University)

  • Zifan Zhang

    (Naval Medical University)

  • Leixin Wei

    (Naval Medical University)

  • Bo Hu

    (Naval Medical University)

  • Hao Zhang

    (967th Hospital of the Joint Logistics Support Force of PLA)

  • Xiaolong Shen

    (Naval Medical University)

  • Yue Wang

    (the Fouth Medical Center of PLA General Hospital)

  • Yang Liu

    (Naval Medical University)

  • Wen Yuan

    (Naval Medical University)

Abstract

Ossification of the posterior longitudinal ligament (OPLL) is an emerging spinal disease caused by heterotopic ossification of the posterior longitudinal ligament. The pathological mechanism is poorly understood, which hinders the development of nonsurgical treatments. Here, we set out to explore the function and mechanism of small extracellular vesicles (sEVs) in OPLL. Global miRNA sequencings are performed on sEVs derived from ligament cells of normal and OPLL patients, and we have showed that miR-320e is abundantly expressed in OPLL-derived sEVs compare to other sEVs. Treatment with either sEVs or miR-320e significantly promote the osteoblastic differentiation of normal longitudinal ligament cells and mesenchymal stem cells and inhibit the osteoclastic differentiation of monocytes. Through a mechanistic study, we find that TAK1 is a downstream target of miR-320e, and we further validate these findings in vivo using OPLL model mice. Together, our data demonstrate that OPLL ligament cells secrete ossification-promoting sEVs that contribute to the development of ossification through the miR-320e/TAK1 axis.

Suggested Citation

  • Chen Xu & Zicheng Zhang & Ning Liu & Li Li & Huajian Zhong & Ruizhe Wang & Qianghui Shi & Zifan Zhang & Leixin Wei & Bo Hu & Hao Zhang & Xiaolong Shen & Yue Wang & Yang Liu & Wen Yuan, 2022. "Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29029-6
    DOI: 10.1038/s41467-022-29029-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-29029-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-29029-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Haiyang Zhang & Ting Deng & Rui Liu & Ming Bai & Likun Zhou & Xia Wang & Shuang Li & Xinyi Wang & Haiou Yang & Jialu Li & Tao Ning & Dingzhi Huang & Hongli Li & Le Zhang & Guoguang Ying & Yi Ba, 2017. "Exosome-delivered EGFR regulates liver microenvironment to promote gastric cancer liver metastasis," Nature Communications, Nature, vol. 8(1), pages 1-11, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ghulam Hassan Dar & Cláudia C. Mendes & Wei-Li Kuan & Alfina A. Speciale & Mariana Conceição & André Görgens & Inna Uliyakina & Miguel J. Lobo & Wooi F. Lim & Samir EL Andaloussi & Imre Mäger & Thomas, 2021. "GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain," Nature Communications, Nature, vol. 12(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29029-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.