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Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading

Author

Listed:
  • Chao Liang

    (Shenzhen University)

  • Qiang Cai

    (Shenzhen University
    Fujian Agriculture and Forestry University)

  • Fei Wang

    (Shenzhen University)

  • Shaofang Li

    (China Agricultural University)

  • Chenjiang You

    (Fudan University
    University of California)

  • Chi Xu

    (Shenzhen University)

  • Lei Gao

    (Shenzhen University)

  • Dechang Cao

    (Shenzhen University)

  • Ting Lan

    (Shenzhen University)

  • Bailong Zhang

    (University of California)

  • Beixin Mo

    (Shenzhen University)

  • Xuemei Chen

    (University of California)

Abstract

MicroRNAs (miRNAs) play crucial roles in gene expression regulation through RNA cleavage or translation repression. Here, we report the identification of an evolutionarily conserved WD40 domain protein as a player in miRNA biogenesis in Arabidopsis thaliana. A mutation in the REDUCTION IN BLEACHED VEIN AREA (RBV) gene encoding a WD40 domain protein led to the suppression of leaf bleaching caused by an artificial miRNA; the mutation also led to a global reduction in the accumulation of endogenous miRNAs. The nuclear protein RBV promotes the transcription of MIR genes into pri-miRNAs by enhancing the occupancy of RNA polymerase II (Pol II) at MIR gene promoters. RBV also promotes the loading of miRNAs into AGO1. In addition, RNA-seq revealed a global splicing defect in the mutant. Thus, this evolutionarily conserved, nuclear WD40 domain protein acts in miRNA biogenesis and RNA splicing.

Suggested Citation

  • Chao Liang & Qiang Cai & Fei Wang & Shaofang Li & Chenjiang You & Chi Xu & Lei Gao & Dechang Cao & Ting Lan & Bailong Zhang & Beixin Mo & Xuemei Chen, 2022. "Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28872-x
    DOI: 10.1038/s41467-022-28872-x
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    References listed on IDEAS

    as
    1. Zhiye Wang & Zeyang Ma & Claudia Castillo-González & Di Sun & Yanjun Li & Bin Yu & Baoyu Zhao & Pingwei Li & Xiuren Zhang, 2018. "SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production," Nature, Nature, vol. 557(7706), pages 516-521, May.
    2. Qiang Cai & Chao Liang & Suikang Wang & Yingnan Hou & Lei Gao & Li Liu & Wenrong He & Wenbo Ma & Beixin Mo & Xuemei Chen, 2018. "The disease resistance protein SNC1 represses the biogenesis of microRNAs and phased siRNAs," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
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    Cited by:

    1. Bangjun Zhou & Huihui Yu & Yong Xue & Mu Li & Chi Zhang & Bin Yu, 2024. "The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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