IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-28677-y.html
   My bibliography  Save this article

Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis

Author

Listed:
  • Shintaro Mise

    (Kyushu University, Fukuoka)

  • Akinobu Matsumoto

    (Kyushu University, Fukuoka)

  • Keisuke Shimada

    (Osaka University)

  • Toshiaki Hosaka

    (RIKEN Center for Biosystems Dynamics Research)

  • Masatomo Takahashi

    (Kyushu University, Fukuoka)

  • Kazuya Ichihara

    (Kyushu University, Fukuoka)

  • Hideyuki Shimizu

    (Kyushu University, Fukuoka
    Tokyo Medical and Dental University)

  • Chisa Shiraishi

    (Kyushu University, Fukuoka)

  • Daisuke Saito

    (Kyushu University, Fukuoka)

  • Mikita Suyama

    (Kyushu University, Fukuoka)

  • Tomoharu Yasuda

    (Hiroshima University, Hiroshima)

  • Toru Ide

    (Okayama University, Okayama)

  • Yoshihiro Izumi

    (Kyushu University, Fukuoka)

  • Takeshi Bamba

    (Kyushu University, Fukuoka)

  • Tomomi Kimura-Someya

    (RIKEN Center for Biosystems Dynamics Research)

  • Mikako Shirouzu

    (RIKEN Center for Biosystems Dynamics Research)

  • Haruhiko Miyata

    (Osaka University)

  • Masahito Ikawa

    (Osaka University
    The University of Tokyo)

  • Keiichi I. Nakayama

    (Kyushu University, Fukuoka)

Abstract

Although several long noncoding RNAs (lncRNAs) have recently been shown to encode small polypeptides, those in testis remain largely uncharacterized. Here we identify two sperm-specific polypeptides, Kastor and Polluks, encoded by a single mouse locus (Gm9999) previously annotated as encoding a lncRNA. Both Kastor and Polluks are inserted in the outer mitochondrial membrane and directly interact with voltage-dependent anion channel (VDAC), despite their different amino acid sequences. Male VDAC3-deficient mice are infertile as a result of reduced sperm motility due to an abnormal mitochondrial sheath in spermatozoa, and deficiency of both Kastor and Polluks also severely impaired male fertility in association with formation of a similarly abnormal mitochondrial sheath. Spermatozoa lacking either Kastor or Polluks partially recapitulate the phenotype of those lacking both. Cooperative function of Kastor and Polluks in regulation of VDAC3 may thus be essential for mitochondrial sheath formation in spermatozoa and for male fertility.

Suggested Citation

  • Shintaro Mise & Akinobu Matsumoto & Keisuke Shimada & Toshiaki Hosaka & Masatomo Takahashi & Kazuya Ichihara & Hideyuki Shimizu & Chisa Shiraishi & Daisuke Saito & Mikita Suyama & Tomoharu Yasuda & To, 2022. "Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28677-y
    DOI: 10.1038/s41467-022-28677-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-28677-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-28677-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Shigeomi Shimizu & Masashi Narita & Yoshihide Tsujimoto & Yoshihide Tsujimoto, 1999. "Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC," Nature, Nature, vol. 399(6735), pages 483-487, June.
    2. Qian Chu & Thomas F. Martinez & Sammy Weiser Novak & Cynthia J. Donaldson & Dan Tan & Joan M. Vaughan & Tina Chang & Jolene K. Diedrich & Leo Andrade & Andrew Kim & Tong Zhang & Uri Manor & Alan Sagha, 2019. "Regulation of the ER stress response by a mitochondrial microprotein," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    3. Akinobu Matsumoto & Alessandra Pasut & Masaki Matsumoto & Riu Yamashita & Jacqueline Fung & Emanuele Monteleone & Alan Saghatelian & Keiichi I. Nakayama & John G. Clohessy & Pier Paolo Pandolfi, 2017. "mTORC1 and muscle regeneration are regulated by the LINC00961-encoded SPAR polypeptide," Nature, Nature, vol. 541(7636), pages 228-232, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ara Lee & Gihyun Sung & Sanghee Shin & Song-Yi Lee & Jaehwan Sim & Truong Thi My Nhung & Tran Diem Nghi & Sang Ki Park & Ponnusamy Pon Sathieshkumar & Imkyeung Kang & Ji Young Mun & Jong-Seo Kim & Hyu, 2024. "OrthoID: profiling dynamic proteomes through time and space using mutually orthogonal chemical tools," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Haipeng Fu & Tingyu Wang & Xiaohui Kong & Kun Yan & Yang Yang & Jingyi Cao & Yafei Yuan & Nan Wang & Kehkooi Kee & Zhi John Lu & Qiaoran Xi, 2022. "A Nodal enhanced micropeptide NEMEP regulates glucose uptake during mesendoderm differentiation of embryonic stem cells," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    3. Claire Grills & Puthen V Jithesh & Jaine Blayney & Shu-Dong Zhang & Dean A Fennell, 2011. "Gene Expression Meta-Analysis Identifies VDAC1 as a Predictor of Poor Outcome in Early Stage Non-Small Cell Lung Cancer," PLOS ONE, Public Library of Science, vol. 6(1), pages 1-8, January.
    4. Yao-Zhong Liu & Yu Zhou & Lei Zhang & Jian Li & Qing Tian & Ji-Gang Zhang & Hong-Wen Deng, 2015. "Attenuated Monocyte Apoptosis, a New Mechanism for Osteoporosis Suggested by a Transcriptome-Wide Expression Study of Monocytes," PLOS ONE, Public Library of Science, vol. 10(2), pages 1-18, February.
    5. Caroline E. Brun & Marie-Claude Sincennes & Alexander Y. T. Lin & Derek Hall & William Jarassier & Peter Feige & Fabien Le Grand & Michael A. Rudnicki, 2022. "GLI3 regulates muscle stem cell entry into GAlert and self-renewal," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    6. Olga Boix & Marion Martinez & Santiago Vidal & Marta Giménez-Alejandre & Lluís Palenzuela & Laura Lorenzo-Sanz & Laura Quevedo & Olivier Moscoso & Jorge Ruiz-Orera & Pilar Ximénez-Embún & Nikaoly Ciri, 2022. "pTINCR microprotein promotes epithelial differentiation and suppresses tumor growth through CDC42 SUMOylation and activation," Nature Communications, Nature, vol. 13(1), pages 1-22, December.
    7. Manna, Smarajit & Banerjee, Jyotirmoy & Ghosh, Subhendu, 2007. "Breathing of voltage dependent anion channel as revealed by the fractal property of its gating," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 386(1), pages 573-580.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28677-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.