IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-28627-8.html
   My bibliography  Save this article

Fast microwave heating-based one-step synthesis of DNA and RNA modified gold nanoparticles

Author

Listed:
  • Mengqi Huang

    (South China Normal University)

  • Erhu Xiong

    (South China Normal University)

  • Yan Wang

    (South China Normal University)

  • Menglu Hu

    (South China Normal University)

  • Huahua Yue

    (South China Normal University)

  • Tian Tian

    (South China Normal University)

  • Debin Zhu

    (South China Normal University)

  • Hong Liu

    (South China Normal University)

  • Xiaoming Zhou

    (South China Normal University)

Abstract

DNA/RNA-gold nanoparticle (DNA/RNA-AuNP) nanoprobes have been widely employed for nanobiotechnology applications. Here, we discover that both thiolated and non-thiolated DNA/RNA can be efficiently attached to AuNPs to achieve high-stable spherical nucleic acid (SNA) within minutes under a domestic microwave (MW)-assisted heating-dry circumstance. Further studies show that for non-thiolated DNA/RNA the conjugation is poly (T/U) tag dependent. Spectroscopy, test strip hybridization, and loading counting experiments indicate that low-affinity poly (T/U) tag mediates the formation of a standing-up conformation, which is distributed in the outer layer of SNA structure. In further application studies, CRISPR/Cas9-sgRNA (136 bp), SARS-CoV-2 RNA fragment (1278 bp), and rolling circle amplification (RCA) DNA products (over 1000 bp) can be successfully attached on AuNPs, which overcomes the routine methods in long-chain nucleic acid-AuNP conjugation, exhibiting great promise in biosensing and nucleic acids delivery applications. Current heating-dry strategy has improved traditional DNA/RNA-AuNP conjugation methods in simplicity, rapidity, cost, and universality.

Suggested Citation

  • Mengqi Huang & Erhu Xiong & Yan Wang & Menglu Hu & Huahua Yue & Tian Tian & Debin Zhu & Hong Liu & Xiaoming Zhou, 2022. "Fast microwave heating-based one-step synthesis of DNA and RNA modified gold nanoparticles," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28627-8
    DOI: 10.1038/s41467-022-28627-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-28627-8
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-28627-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Mark J. Mulligan & Kirsten E. Lyke & Nicholas Kitchin & Judith Absalon & Alejandra Gurtman & Stephen Lockhart & Kathleen Neuzil & Vanessa Raabe & Ruth Bailey & Kena A. Swanson & Ping Li & Kenneth Kour, 2020. "Phase I/II study of COVID-19 RNA vaccine BNT162b1 in adults," Nature, Nature, vol. 586(7830), pages 589-593, October.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yang Liu & Xianwen Zhang & Jianying Liu & Hongjie Xia & Jing Zou & Antonio E. Muruato & Sivakumar Periasamy & Chaitanya Kurhade & Jessica A. Plante & Nathen E. Bopp & Birte Kalveram & Alexander Bukrey, 2022. "A live-attenuated SARS-CoV-2 vaccine candidate with accessory protein deletions," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Chaitanya Kurhade & Jing Zou & Hongjie Xia & Hui Cai & Qi Yang & Mark Cutler & David Cooper & Alexander Muik & Kathrin U. Jansen & Xuping Xie & Kena A. Swanson & Pei‑Yong Shi, 2022. "Neutralization of Omicron BA.1, BA.2, and BA.3 SARS-CoV-2 by 3 doses of BNT162b2 vaccine," Nature Communications, Nature, vol. 13(1), pages 1-4, December.
    3. Wu, Yan Yan & Walkover, Margaret & Zhang, Wei, 2021. "Trends in psychological distress and COVID-19 incidence across 15 U.S. metropolitan statistical areas in 2020," Social Science & Medicine, Elsevier, vol. 285(C).

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28627-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.