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Fast microwave heating-based one-step synthesis of DNA and RNA modified gold nanoparticles

Author

Listed:
  • Mengqi Huang

    (South China Normal University)

  • Erhu Xiong

    (South China Normal University)

  • Yan Wang

    (South China Normal University)

  • Menglu Hu

    (South China Normal University)

  • Huahua Yue

    (South China Normal University)

  • Tian Tian

    (South China Normal University)

  • Debin Zhu

    (South China Normal University)

  • Hong Liu

    (South China Normal University)

  • Xiaoming Zhou

    (South China Normal University)

Abstract

DNA/RNA-gold nanoparticle (DNA/RNA-AuNP) nanoprobes have been widely employed for nanobiotechnology applications. Here, we discover that both thiolated and non-thiolated DNA/RNA can be efficiently attached to AuNPs to achieve high-stable spherical nucleic acid (SNA) within minutes under a domestic microwave (MW)-assisted heating-dry circumstance. Further studies show that for non-thiolated DNA/RNA the conjugation is poly (T/U) tag dependent. Spectroscopy, test strip hybridization, and loading counting experiments indicate that low-affinity poly (T/U) tag mediates the formation of a standing-up conformation, which is distributed in the outer layer of SNA structure. In further application studies, CRISPR/Cas9-sgRNA (136 bp), SARS-CoV-2 RNA fragment (1278 bp), and rolling circle amplification (RCA) DNA products (over 1000 bp) can be successfully attached on AuNPs, which overcomes the routine methods in long-chain nucleic acid-AuNP conjugation, exhibiting great promise in biosensing and nucleic acids delivery applications. Current heating-dry strategy has improved traditional DNA/RNA-AuNP conjugation methods in simplicity, rapidity, cost, and universality.

Suggested Citation

  • Mengqi Huang & Erhu Xiong & Yan Wang & Menglu Hu & Huahua Yue & Tian Tian & Debin Zhu & Hong Liu & Xiaoming Zhou, 2022. "Fast microwave heating-based one-step synthesis of DNA and RNA modified gold nanoparticles," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28627-8
    DOI: 10.1038/s41467-022-28627-8
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    1. Mark J. Mulligan & Kirsten E. Lyke & Nicholas Kitchin & Judith Absalon & Alejandra Gurtman & Stephen Lockhart & Kathleen Neuzil & Vanessa Raabe & Ruth Bailey & Kena A. Swanson & Ping Li & Kenneth Kour, 2020. "Phase I/II study of COVID-19 RNA vaccine BNT162b1 in adults," Nature, Nature, vol. 586(7830), pages 589-593, October.
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