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Kaposi’s sarcoma-associated herpesvirus T cell responses in HIV seronegative individuals from rural Uganda

Author

Listed:
  • Angela Nalwoga

    (MRC/UVRI and LSHTM Uganda Research Unit
    University of Colorado, Anschutz Medical Campus)

  • Romin Roshan

    (Frederick National Laboratory for Cancer Research)

  • Kyle Moore

    (Frederick National Laboratory for Cancer Research)

  • Vickie Marshall

    (Frederick National Laboratory for Cancer Research)

  • Wendell Miley

    (Frederick National Laboratory for Cancer Research)

  • Nazzarena Labo

    (Frederick National Laboratory for Cancer Research)

  • Marjorie Nakibuule

    (MRC/UVRI and LSHTM Uganda Research Unit)

  • Stephen Cose

    (MRC/UVRI and LSHTM Uganda Research Unit
    London School of Hygiene & Tropical Medicine)

  • Rosemary Rochford

    (University of Colorado, Anschutz Medical Campus)

  • Robert Newton

    (University of York)

  • Denise Whitby

    (Frederick National Laboratory for Cancer Research)

Abstract

T cell responses to Kaposi’s sarcoma-associated herpesvirus (KSHV) are likely essential in the control of KSHV infection and protection from associated disease, but remain poorly characterised. KSHV prevalence in rural Uganda is high at >90%. Here we investigate IFN- γ T cell responses to the KSHV proteome in HIV-negative individuals from a rural Ugandan population. We use an ex-vivo IFN- γ ELISpot assay with overlapping peptide pools spanning 83 KSHV open reading frames (ORF) on peripheral blood mononuclear cells (PBMC) from 116 individuals. KSHV-specific T cell IFN- γ responses are of low intensity and heterogeneous, with no evidence of immune dominance; by contrast, IFN- γ responses to Epstein–Barr virus, Cytomegalovirus and influenza peptides are frequent and intense. Individuals with KSHV DNA in PBMC have higher IFN- γ responses to ORF73 (p = 0.02) and lower responses to K8.1 (p = 0.004) when compared with those without KSHV DNA. In summary, we demonstrate low intensity, heterogeneous T cell responses to KSHV in immune-competent individuals.

Suggested Citation

  • Angela Nalwoga & Romin Roshan & Kyle Moore & Vickie Marshall & Wendell Miley & Nazzarena Labo & Marjorie Nakibuule & Stephen Cose & Rosemary Rochford & Robert Newton & Denise Whitby, 2021. "Kaposi’s sarcoma-associated herpesvirus T cell responses in HIV seronegative individuals from rural Uganda," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27623-8
    DOI: 10.1038/s41467-021-27623-8
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    Cited by:

    1. Nicole Caduff & Lisa Rieble & Michelle Böni & Donal McHugh & Romin Roshan & Wendell Miley & Nazzarena Labo & Sumanta Barman & Matthew Trivett & Douwe M. T. Bosma & Julia Rühl & Norbert Goebels & Denis, 2024. "KSHV infection of B cells primes protective T cell responses in humanized mice," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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