IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-27599-5.html
   My bibliography  Save this article

Integrated single-cell transcriptome analysis reveals heterogeneity of esophageal squamous cell carcinoma microenvironment

Author

Listed:
  • Huy Q. Dinh

    (University of Wisconsin-Madison School of Medicine and Public Health
    La Jolla Institute for Immunology)

  • Feng Pan

    (Shantou University Medical College
    Guangdong Esophageal Cancer Research Institute, Shantou Sub-center)

  • Geng Wang

    (Guangdong Esophageal Cancer Research Institute, Shantou Sub-center
    Cancer Hospital of Shantou University Medical College)

  • Qing-Feng Huang

    (Shantou University Medical College
    Guangdong Esophageal Cancer Research Institute, Shantou Sub-center)

  • Claire E. Olingy

    (La Jolla Institute for Immunology)

  • Zhi-Yong Wu

    (Shantou Central Hospital)

  • Shao-Hong Wang

    (Shantou Central Hospital)

  • Xin Xu

    (Shantou University Medical College
    Guangdong Esophageal Cancer Research Institute, Shantou Sub-center)

  • Xiu-E Xu

    (Shantou University Medical College
    Guangdong Esophageal Cancer Research Institute, Shantou Sub-center)

  • Jian-Zhong He

    (Shantou University Medical College
    Guangdong Esophageal Cancer Research Institute, Shantou Sub-center)

  • Qian Yang

    (Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center)

  • Sandra Orsulic

    (Cedars-Sinai Medical Center, David Geffen School of Medicine at UCLA)

  • Marcela Haro

    (Cedars-Sinai Medical Center, David Geffen School of Medicine at UCLA)

  • Li-Yan Li

    (Shantou University Medical College)

  • Guo-Wei Huang

    (Shantou University Medical College)

  • Joshua J. Breunig

    (Cedars-Sinai Medical Center)

  • H. Phillip Koeffler

    (Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center)

  • Catherine C. Hedrick

    (La Jolla Institute for Immunology)

  • Li-Yan Xu

    (Shantou University Medical College
    Guangdong Esophageal Cancer Research Institute, Shantou Sub-center)

  • De-Chen Lin

    (Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center)

  • En-Min Li

    (Shantou University Medical College
    Guangdong Esophageal Cancer Research Institute, Shantou Sub-center)

Abstract

The tumor microenvironment is a highly complex ecosystem of diverse cell types, which shape cancer biology and impact the responsiveness to therapy. Here, we analyze the microenvironment of esophageal squamous cell carcinoma (ESCC) using single-cell transcriptome sequencing in 62,161 cells from blood, adjacent nonmalignant and matched tumor samples from 11 ESCC patients. We uncover heterogeneity in most cell types of the ESCC stroma, particularly in the fibroblast and immune cell compartments. We identify a tumor-specific subset of CST1+ myofibroblasts with prognostic values and potential biological significance. CST1+ myofibroblasts are also highly tumor-specific in other cancer types. Additionally, a subset of antigen-presenting fibroblasts is revealed and validated. Analyses of myeloid and T lymphoid lineages highlight the immunosuppressive nature of the ESCC microenvironment, and identify cancer-specific expression of immune checkpoint inhibitors. This work establishes a rich resource of stromal cell types of the ESCC microenvironment for further understanding of ESCC biology.

Suggested Citation

  • Huy Q. Dinh & Feng Pan & Geng Wang & Qing-Feng Huang & Claire E. Olingy & Zhi-Yong Wu & Shao-Hong Wang & Xin Xu & Xiu-E Xu & Jian-Zhong He & Qian Yang & Sandra Orsulic & Marcela Haro & Li-Yan Li & Guo, 2021. "Integrated single-cell transcriptome analysis reveals heterogeneity of esophageal squamous cell carcinoma microenvironment," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27599-5
    DOI: 10.1038/s41467-021-27599-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-27599-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-27599-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. De-Chen Lin, 2023. "Large-scale genomic analyses reveal alterations and mechanisms underlying clonal evolution and immune evasion in esophageal cancer," Nature Communications, Nature, vol. 14(1), pages 1-3, December.
    2. Yuan Jiang & Yueyuan Zheng & Yuan-Wei Zhang & Shuai Kong & Jinxiu Dong & Fei Wang & Benjamin Ziman & Sigal Gery & Jia-Jie Hao & Dan Zhou & Jianian Zhou & Allen S. Ho & Uttam K. Sinha & Jian Chen & Shu, 2024. "Reciprocal inhibition between TP63 and STAT1 regulates anti-tumor immune response through interferon-γ signaling in squamous cancer," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27599-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.