IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-26837-0.html
   My bibliography  Save this article

Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution

Author

Listed:
  • Gareth O. S. Williams

    (University of Edinburgh)

  • Elvira Williams

    (Durham University)

  • Neil Finlayson

    (University of Edinburgh, King’s Buildings)

  • Ahmet T. Erdogan

    (University of Edinburgh, King’s Buildings)

  • Qiang Wang

    (University of Edinburgh)

  • Susan Fernandes

    (University of Edinburgh)

  • Ahsan R. Akram

    (University of Edinburgh)

  • Kev Dhaliwal

    (University of Edinburgh)

  • Robert K. Henderson

    (University of Edinburgh, King’s Buildings)

  • John M. Girkin

    (Durham University)

  • Mark Bradley

    (University of Edinburgh)

Abstract

The use of optical techniques to interrogate wide ranging samples from semiconductors to biological tissue for rapid analysis and diagnostics has gained wide adoption over the past decades. The desire to collect ever more spatially, spectrally and temporally detailed optical signatures for sample characterization has specifically driven a sharp rise in new optical microscopy technologies. Here we present a high-speed optical scanning microscope capable of capturing time resolved images across 512 spectral and 32 time channels in a single acquisition with the potential for ~0.2 frames per second (256 × 256 image pixels). Each pixel in the resulting images contains a detailed data cube for the study of diverse time resolved light driven phenomena. This is enabled by integration of system control electronics and on-chip processing which overcomes the challenges presented by high data volume and low imaging speed, often bottlenecks in previous systems.

Suggested Citation

  • Gareth O. S. Williams & Elvira Williams & Neil Finlayson & Ahmet T. Erdogan & Qiang Wang & Susan Fernandes & Ahsan R. Akram & Kev Dhaliwal & Robert K. Henderson & John M. Girkin & Mark Bradley, 2021. "Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26837-0
    DOI: 10.1038/s41467-021-26837-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-26837-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-26837-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Wiebke Jahr & Benjamin Schmid & Christopher Schmied & Florian O. Fahrbach & Jan Huisken, 2015. "Hyperspectral light sheet microscopy," Nature Communications, Nature, vol. 6(1), pages 1-7, November.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Xiaomeng Han & Xiaotang Lu & Peter H. Li & Shuohong Wang & Richard Schalek & Yaron Meirovitch & Zudi Lin & Jason Adhinarta & Karl D. Murray & Leah M. MacNiven & Daniel R. Berger & Yuelong Wu & Tao Fan, 2024. "Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    2. Jonghee Yoon & Alexandru Grigoroiu & Sarah E Bohndiek, 2020. "A background correction method to compensate illumination variation in hyperspectral imaging," PLOS ONE, Public Library of Science, vol. 15(3), pages 1-21, March.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26837-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.