IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-26826-3.html
   My bibliography  Save this article

CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes

Author

Listed:
  • Dalileh Nabi

    (Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden
    Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health)

  • Hauke Drechsler

    (B CUBE-Center for Molecular Bioengineering, Technische Universität Dresden)

  • Johannes Pschirer

    (Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden)

  • Franz Korn

    (Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden)

  • Nadine Schuler

    (Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden)

  • Stefan Diez

    (B CUBE-Center for Molecular Bioengineering, Technische Universität Dresden
    Cluster of Excellence Physics of Life, Technische Universität Dresden
    Max Planck Institute of Molecular Cell Biology and Genetics)

  • Rolf Jessberger

    (Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden)

  • Mariola Chacón

    (Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden
    CABIMER, Centro Andaluz de Biología Molecular & Medicina Regenerativa)

Abstract

Proper chromosome segregation is essential to avoid aneuploidy, yet this process fails with increasing age in mammalian oocytes. Here we report a role for the scarcely described protein CENP-V in oocyte spindle formation and chromosome segregation. We show that depending on the oocyte maturation state, CENP-V localizes to centromeres, to microtubule organizing centers, and to spindle microtubules. We find that Cenp-V−/− oocytes feature severe deficiencies, including metaphase I arrest, strongly reduced polar body extrusion, increased numbers of mis-aligned chromosomes and aneuploidy, multipolar spindles, unfocused spindle poles and loss of kinetochore spindle fibres. We also show that CENP-V protein binds, diffuses along, and bundles microtubules in vitro. The spindle assembly checkpoint arrests about half of metaphase I Cenp-V−/− oocytes from young adults only. This finding suggests checkpoint weakening in ageing oocytes, which mature despite carrying mis-aligned chromosomes. Thus, CENP-V is a microtubule bundling protein crucial to faithful oocyte meiosis, and Cenp-V−/− oocytes reveal age-dependent weakening of the spindle assembly checkpoint.

Suggested Citation

  • Dalileh Nabi & Hauke Drechsler & Johannes Pschirer & Franz Korn & Nadine Schuler & Stefan Diez & Rolf Jessberger & Mariola Chacón, 2021. "CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26826-3
    DOI: 10.1038/s41467-021-26826-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-26826-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-26826-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Dean Clift & Melina Schuh, 2015. "A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes," Nature Communications, Nature, vol. 6(1), pages 1-12, November.
    2. Janko Kajtez & Anastasia Solomatina & Maja Novak & Bruno Polak & Kruno Vukušić & Jonas Rüdiger & Gheorghe Cojoc & Ana Milas & Ivana Šumanovac Šestak & Patrik Risteski & Federica Tavano & Anna H. Klemm, 2016. "Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores," Nature Communications, Nature, vol. 7(1), pages 1-11, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ai Kiyomitsu & Toshiya Nishimura & Shiang Jyi Hwang & Satoshi Ansai & Masato T. Kanemaki & Minoru Tanaka & Tomomi Kiyomitsu, 2024. "Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    2. Sushil Khanal & Ankit Jaiswal & Rajanikanth Chowdanayaka & Nahshon Puente & Katerina Turner & Kebron Yeshitela Assefa & Mohamad Nawras & Ezekiel David Back & Abigail Royfman & James P. Burkett & Soon , 2024. "The evolution of centriole degradation in mouse sperm," Nature Communications, Nature, vol. 15(1), pages 1-22, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26826-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.