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Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms

Author

Listed:
  • Nicolas Flaugnatti

    (Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Sandrine Isaac

    (Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Leonardo F. Lemos Rocha

    (Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Sandrine Stutzmann

    (Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Olaya Rendueles

    (Institut Pasteur, CNRS, UMR3525)

  • Candice Stoudmann

    (Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Nina Vesel

    (Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Marc Garcia-Garcera

    (University of Lausanne)

  • Amandine Buffet

    (Institut Pasteur, CNRS, UMR3525)

  • Thibault G. Sana

    (Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)
    LIPME, Université de Toulouse, INRAE, CNRS)

  • Eduardo P. C. Rocha

    (Institut Pasteur, CNRS, UMR3525)

  • Melanie Blokesch

    (Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL))

Abstract

While the major virulence factors for Vibrio cholerae, the cause of the devastating diarrheal disease cholera, have been extensively studied, the initial intestinal colonization of the bacterium is not well understood because non-human adult animals are refractory to its colonization. Recent studies suggest the involvement of an interbacterial killing device known as the type VI secretion system (T6SS). Here, we tested the T6SS-dependent interaction of V. cholerae with a selection of human gut commensal isolates. We show that the pathogen efficiently depleted representative genera of the Proteobacteria in vitro, while members of the Enterobacter cloacae complex and several Klebsiella species remained unaffected. We demonstrate that this resistance against T6SS assaults was mediated by the production of superior T6SS machinery or a barrier exerted by group I capsules. Collectively, our data provide new insights into immunity protein-independent T6SS resistance employed by the human microbiota and colonization resistance in general.

Suggested Citation

  • Nicolas Flaugnatti & Sandrine Isaac & Leonardo F. Lemos Rocha & Sandrine Stutzmann & Olaya Rendueles & Candice Stoudmann & Nina Vesel & Marc Garcia-Garcera & Amandine Buffet & Thibault G. Sana & Eduar, 2021. "Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26041-0
    DOI: 10.1038/s41467-021-26041-0
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    Cited by:

    1. Amandine Nucci & Eduardo P. C. Rocha & Olaya Rendueles, 2022. "Adaptation to novel spatially-structured environments is driven by the capsule and alters virulence-associated traits," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Matthieu Haudiquet & Julie Bris & Amandine Nucci & Rémy A. Bonnin & Pilar Domingo-Calap & Eduardo P. C. Rocha & Olaya Rendueles, 2024. "Capsules and their traits shape phage susceptibility and plasmid conjugation efficiency," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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