IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-25909-5.html
   My bibliography  Save this article

Direct photoresponsive inhibition of a p53-like transcription activation domain in PIF3 by Arabidopsis phytochrome B

Author

Listed:
  • Chan Yul Yoo

    (University of California)

  • Jiangman He

    (University of California)

  • Qing Sang

    (University of California
    Shenzhen University)

  • Yongjian Qiu

    (University of California)

  • Lingyun Long

    (University of California)

  • Ruth Jean-Ae Kim

    (University of California)

  • Emily G. Chong

    (University of California)

  • Joseph Hahm

    (University of California)

  • Nicholas Morffy

    (Duke University)

  • Pei Zhou

    (Duke University School of Medicine)

  • Lucia C. Strader

    (Duke University)

  • Akira Nagatani

    (Kyoto University)

  • Beixin Mo

    (Shenzhen University)

  • Xuemei Chen

    (University of California)

  • Meng Chen

    (University of California)

Abstract

Photoactivated phytochrome B (PHYB) binds to antagonistically acting PHYTOCHROME-INTERACTING transcription FACTORs (PIFs) to regulate hundreds of light responsive genes in Arabidopsis by promoting PIF degradation. However, whether PHYB directly controls the transactivation activity of PIFs remains ambiguous. Here we show that the prototypic PIF, PIF3, possesses a p53-like transcription activation domain (AD) consisting of a hydrophobic activator motif flanked by acidic residues. A PIF3mAD mutant, in which the activator motif is replaced with alanines, fails to activate PIF3 target genes in Arabidopsis, validating the functions of the PIF3 AD in vivo. Intriguingly, the N-terminal photosensory module of PHYB binds immediately adjacent to the PIF3 AD to repress PIF3’s transactivation activity, demonstrating a novel PHYB signaling mechanism through direct interference of the transactivation activity of PIF3. Our findings indicate that PHYB, likely also PHYA, controls the stability and activity of PIFs via structurally separable dual signaling mechanisms.

Suggested Citation

  • Chan Yul Yoo & Jiangman He & Qing Sang & Yongjian Qiu & Lingyun Long & Ruth Jean-Ae Kim & Emily G. Chong & Joseph Hahm & Nicholas Morffy & Pei Zhou & Lucia C. Strader & Akira Nagatani & Beixin Mo & Xu, 2021. "Direct photoresponsive inhibition of a p53-like transcription activation domain in PIF3 by Arabidopsis phytochrome B," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25909-5
    DOI: 10.1038/s41467-021-25909-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-25909-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-25909-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Qing Sang & Lusheng Fan & Tianxiang Liu & Yongjian Qiu & Juan Du & Beixin Mo & Meng Chen & Xuemei Chen, 2023. "MicroRNA156 conditions auxin sensitivity to enable growth plasticity in response to environmental changes in Arabidopsis," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Ruth Jean Ae Kim & De Fan & Jiangman He & Keunhwa Kim & Juan Du & Meng Chen, 2024. "Photobody formation spatially segregates two opposing phytochrome B signaling actions of PIF5 degradation and stabilization," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25909-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.