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Direct photoresponsive inhibition of a p53-like transcription activation domain in PIF3 by Arabidopsis phytochrome B

Author

Listed:
  • Chan Yul Yoo

    (University of California)

  • Jiangman He

    (University of California)

  • Qing Sang

    (University of California
    Shenzhen University)

  • Yongjian Qiu

    (University of California)

  • Lingyun Long

    (University of California)

  • Ruth Jean-Ae Kim

    (University of California)

  • Emily G. Chong

    (University of California)

  • Joseph Hahm

    (University of California)

  • Nicholas Morffy

    (Duke University)

  • Pei Zhou

    (Duke University School of Medicine)

  • Lucia C. Strader

    (Duke University)

  • Akira Nagatani

    (Kyoto University)

  • Beixin Mo

    (Shenzhen University)

  • Xuemei Chen

    (University of California)

  • Meng Chen

    (University of California)

Abstract

Photoactivated phytochrome B (PHYB) binds to antagonistically acting PHYTOCHROME-INTERACTING transcription FACTORs (PIFs) to regulate hundreds of light responsive genes in Arabidopsis by promoting PIF degradation. However, whether PHYB directly controls the transactivation activity of PIFs remains ambiguous. Here we show that the prototypic PIF, PIF3, possesses a p53-like transcription activation domain (AD) consisting of a hydrophobic activator motif flanked by acidic residues. A PIF3mAD mutant, in which the activator motif is replaced with alanines, fails to activate PIF3 target genes in Arabidopsis, validating the functions of the PIF3 AD in vivo. Intriguingly, the N-terminal photosensory module of PHYB binds immediately adjacent to the PIF3 AD to repress PIF3’s transactivation activity, demonstrating a novel PHYB signaling mechanism through direct interference of the transactivation activity of PIF3. Our findings indicate that PHYB, likely also PHYA, controls the stability and activity of PIFs via structurally separable dual signaling mechanisms.

Suggested Citation

  • Chan Yul Yoo & Jiangman He & Qing Sang & Yongjian Qiu & Lingyun Long & Ruth Jean-Ae Kim & Emily G. Chong & Joseph Hahm & Nicholas Morffy & Pei Zhou & Lucia C. Strader & Akira Nagatani & Beixin Mo & Xu, 2021. "Direct photoresponsive inhibition of a p53-like transcription activation domain in PIF3 by Arabidopsis phytochrome B," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25909-5
    DOI: 10.1038/s41467-021-25909-5
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    Cited by:

    1. Qing Sang & Lusheng Fan & Tianxiang Liu & Yongjian Qiu & Juan Du & Beixin Mo & Meng Chen & Xuemei Chen, 2023. "MicroRNA156 conditions auxin sensitivity to enable growth plasticity in response to environmental changes in Arabidopsis," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Ruth Jean Ae Kim & De Fan & Jiangman He & Keunhwa Kim & Juan Du & Meng Chen, 2024. "Photobody formation spatially segregates two opposing phytochrome B signaling actions of PIF5 degradation and stabilization," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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