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Cryo-EM structure of the mature and infective Mayaro virus at 4.4 Å resolution reveals features of arthritogenic alphaviruses

Author

Listed:
  • Helder V. Ribeiro-Filho

    (Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Lais D. Coimbra

    (Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Alexandre Cassago

    (Brazilian Nanotechnology National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Rebeca P. F. Rocha

    (Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • João Victor da Silva Guerra

    (Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Rafael Felicio

    (Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Carolina Moretto Carnieli

    (Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Luiza Leme

    (Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Antonio Cláudio Padilha

    (Brazilian Nanotechnology National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Adriana F. Paes Leme

    (Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Daniela B. B. Trivella

    (Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Rodrigo Villares Portugal

    (Brazilian Nanotechnology National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Paulo Sérgio Lopes-de-Oliveira

    (Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

  • Rafael Elias Marques

    (Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials (CNPEM))

Abstract

Mayaro virus (MAYV) is an emerging arbovirus of the Americas that may cause a debilitating arthritogenic disease. The biology of MAYV is not fully understood and largely inferred from related arthritogenic alphaviruses. Here, we present the structure of MAYV at 4.4 Å resolution, obtained from a preparation of mature, infective virions. MAYV presents typical alphavirus features and organization. Interactions between viral proteins that lead to particle formation are described together with a hydrophobic pocket formed between E1 and E2 spike proteins and conformational epitopes specific of MAYV. We also describe MAYV glycosylation residues in E1 and E2 that may affect MXRA8 host receptor binding, and a molecular “handshake” between MAYV spikes formed by N262 glycosylation in adjacent E2 proteins. The structure of MAYV is suggestive of structural and functional complexity among alphaviruses, which may be targeted for specificity or antiviral activity.

Suggested Citation

  • Helder V. Ribeiro-Filho & Lais D. Coimbra & Alexandre Cassago & Rebeca P. F. Rocha & João Victor da Silva Guerra & Rafael Felicio & Carolina Moretto Carnieli & Luiza Leme & Antonio Cláudio Padilha & A, 2021. "Cryo-EM structure of the mature and infective Mayaro virus at 4.4 Å resolution reveals features of arthritogenic alphaviruses," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23400-9
    DOI: 10.1038/s41467-021-23400-9
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    Cited by:

    1. Ningning Wang & Andres Merits & Michael Veit & Laura Sandra Lello & Shuhan Kong & Houqi Jiao & Jie Chen & Yu Wang & Georgi Dobrikov & Félix A. Rey & Shuo Su, 2024. "LDL receptor in alphavirus entry: structural analysis and implications for antiviral therapy," Nature Communications, Nature, vol. 15(1), pages 1-7, December.

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