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A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

Author

Listed:
  • Guanhua Xun

    (University of Illinois at Urbana-Champaign)

  • Stephan Thomas Lane

    (Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign)

  • Vassily Andrew Petrov

    (Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign)

  • Brandon Elliott Pepa

    (University of Illinois at Urbana-Champaign)

  • Huimin Zhao

    (University of Illinois at Urbana-Champaign
    Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign
    University of Illinois at Urbana-Champaign)

Abstract

The need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.

Suggested Citation

  • Guanhua Xun & Stephan Thomas Lane & Vassily Andrew Petrov & Brandon Elliott Pepa & Huimin Zhao, 2021. "A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23185-x
    DOI: 10.1038/s41467-021-23185-x
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    Cited by:

    1. Hu Li & Huarui Gong & Tsz Hung Wong & Jingkun Zhou & Yuqiong Wang & Long Lin & Ying Dou & Huiling Jia & Xingcan Huang & Zhan Gao & Rui Shi & Ya Huang & Zhenlin Chen & Wooyoung PARK & Ji Yu Li & Hongwe, 2023. "Wireless, battery-free, multifunctional integrated bioelectronics for respiratory pathogens monitoring and severity evaluation," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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