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The double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1

Author

Listed:
  • Sabrina Dietz

    (Institute of Molecular Biology (IMB))

  • Miguel Vasconcelos Almeida

    (Institute of Molecular Biology (IMB)
    University of Cambridge
    University of Cambridge)

  • Emily Nischwitz

    (Institute of Molecular Biology (IMB))

  • Jan Schreier

    (Institute of Molecular Biology (IMB))

  • Nikenza Viceconte

    (Institute of Molecular Biology (IMB))

  • Albert Fradera-Sola

    (Institute of Molecular Biology (IMB))

  • Christian Renz

    (Institute of Molecular Biology (IMB))

  • Alejandro Ceron-Noriega

    (Institute of Molecular Biology (IMB))

  • Helle D. Ulrich

    (Institute of Molecular Biology (IMB))

  • Dennis Kappei

    (National University of Singapore
    National University of Singapore)

  • René F. Ketting

    (Institute of Molecular Biology (IMB))

  • Falk Butter

    (Institute of Molecular Biology (IMB))

Abstract

Telomeres are bound by dedicated proteins, which protect them from DNA damage and regulate telomere length homeostasis. In the nematode Caenorhabditis elegans, a comprehensive understanding of the proteins interacting with the telomere sequence is lacking. Here, we harnessed a quantitative proteomics approach to identify TEBP-1 and TEBP-2, two paralogs expressed in the germline and embryogenesis that associate to telomeres in vitro and in vivo. tebp-1 and tebp-2 mutants display strikingly distinct phenotypes: tebp-1 mutants have longer telomeres than wild-type animals, while tebp-2 mutants display shorter telomeres and a Mortal Germline. Notably, tebp-1;tebp-2 double mutant animals have synthetic sterility, with germlines showing signs of severe mitotic and meiotic arrest. Furthermore, we show that POT-1 forms a telomeric complex with TEBP-1 and TEBP-2, which bridges TEBP-1/-2 with POT-2/MRT-1. These results provide insights into the composition and organization of a telomeric protein complex in C. elegans.

Suggested Citation

  • Sabrina Dietz & Miguel Vasconcelos Almeida & Emily Nischwitz & Jan Schreier & Nikenza Viceconte & Albert Fradera-Sola & Christian Renz & Alejandro Ceron-Noriega & Helle D. Ulrich & Dennis Kappei & Ren, 2021. "The double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1," Nature Communications, Nature, vol. 12(1), pages 1-20, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22861-2
    DOI: 10.1038/s41467-021-22861-2
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    Cited by:

    1. Dadong Dai & Chuanshuai Xie & Yayi Zhou & Dexin Bo & Shurong Zhang & Shengqiang Mao & Yucheng Liao & Simeng Cui & Zhaolu Zhu & Xueyu Wang & Fanling Li & Donghai Peng & Jinshui Zheng & Ming Sun, 2023. "Unzipped chromosome-level genomes reveal allopolyploid nematode origin pattern as unreduced gamete hybridization," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    2. Ana Paula Zotta Mota & Georgios D. Koutsovoulos & Laetitia Perfus-Barbeoch & Evelin Despot-Slade & Karine Labadie & Jean-Marc Aury & Karine Robbe-Sermesant & Marc Bailly-Bechet & Caroline Belser & Art, 2024. "Unzipped genome assemblies of polyploid root-knot nematodes reveal unusual and clade-specific telomeric repeats," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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