IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-22308-8.html
   My bibliography  Save this article

Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technology

Author

Listed:
  • Keman Cheng

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences
    Xiamen University)

  • Ruifang Zhao

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Yao Li

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences
    Xiamen University)

  • Yingqiu Qi

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Yazhou Wang

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Yinlong Zhang

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Hao Qin

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Yuting Qin

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Long Chen

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Chen Li

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Jie Liang

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Yujing Li

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Jiaqi Xu

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Xuexiang Han

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Gregory J. Anderson

    (Iron Metabolism Laboratory, QIMR Berghofer Medical Research Institute)

  • Jian Shi

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Lei Ren

    (Xiamen University)

  • Xiao Zhao

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Guangjun Nie

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

Abstract

An effective tumor vaccine vector that can rapidly display neoantigens is urgently needed. Outer membrane vesicles (OMVs) can strongly activate the innate immune system and are qualified as immunoadjuvants. Here, we describe a versatile OMV-based vaccine platform to elicit a specific anti-tumor immune response via specifically presenting antigens onto OMV surface. We first display tumor antigens on the OMVs surface by fusing with ClyA protein, and then simplify the antigen display process by employing a Plug-and-Display system comprising the tag/catcher protein pairs. OMVs decorated with different protein catchers can simultaneously display multiple, distinct tumor antigens to elicit a synergistic antitumour immune response. In addition, the bioengineered OMVs loaded with different tumor antigens can abrogate lung melanoma metastasis and inhibit subcutaneous colorectal cancer growth. The ability of the bioengineered OMV-based platform to rapidly and simultaneously display antigens may facilitate the development of these agents for personalized tumour vaccines.

Suggested Citation

  • Keman Cheng & Ruifang Zhao & Yao Li & Yingqiu Qi & Yazhou Wang & Yinlong Zhang & Hao Qin & Yuting Qin & Long Chen & Chen Li & Jie Liang & Yujing Li & Jiaqi Xu & Xuexiang Han & Gregory J. Anderson & Ji, 2021. "Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technology," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22308-8
    DOI: 10.1038/s41467-021-22308-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-22308-8
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-22308-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Dinh-Huy Nguyen & Sung-Hwan You & Hien Thi-Thu Ngo & Khuynh Nguyen & Khang Vuong Tran & Tan-Huy Chu & So-young Kim & Sang-Jun Ha & Yeongjin Hong & Jung-Joon Min, 2024. "Reprogramming the tumor immune microenvironment using engineered dual-drug loaded Salmonella," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Dinh-Huy Nguyen & Ari Chong & Yeongjin Hong & Jung-Joon Min, 2023. "Bioengineering of bacteria for cancer immunotherapy," Nature Communications, Nature, vol. 14(1), pages 1-5, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22308-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.