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Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technology

Author

Listed:
  • Keman Cheng

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences
    Xiamen University)

  • Ruifang Zhao

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Yao Li

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences
    Xiamen University)

  • Yingqiu Qi

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Yazhou Wang

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Yinlong Zhang

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Hao Qin

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Yuting Qin

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Long Chen

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Chen Li

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Jie Liang

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Yujing Li

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Jiaqi Xu

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Xuexiang Han

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Gregory J. Anderson

    (Iron Metabolism Laboratory, QIMR Berghofer Medical Research Institute)

  • Jian Shi

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Lei Ren

    (Xiamen University)

  • Xiao Zhao

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

  • Guangjun Nie

    (CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China
    University of Chinese Academy of Sciences)

Abstract

An effective tumor vaccine vector that can rapidly display neoantigens is urgently needed. Outer membrane vesicles (OMVs) can strongly activate the innate immune system and are qualified as immunoadjuvants. Here, we describe a versatile OMV-based vaccine platform to elicit a specific anti-tumor immune response via specifically presenting antigens onto OMV surface. We first display tumor antigens on the OMVs surface by fusing with ClyA protein, and then simplify the antigen display process by employing a Plug-and-Display system comprising the tag/catcher protein pairs. OMVs decorated with different protein catchers can simultaneously display multiple, distinct tumor antigens to elicit a synergistic antitumour immune response. In addition, the bioengineered OMVs loaded with different tumor antigens can abrogate lung melanoma metastasis and inhibit subcutaneous colorectal cancer growth. The ability of the bioengineered OMV-based platform to rapidly and simultaneously display antigens may facilitate the development of these agents for personalized tumour vaccines.

Suggested Citation

  • Keman Cheng & Ruifang Zhao & Yao Li & Yingqiu Qi & Yazhou Wang & Yinlong Zhang & Hao Qin & Yuting Qin & Long Chen & Chen Li & Jie Liang & Yujing Li & Jiaqi Xu & Xuexiang Han & Gregory J. Anderson & Ji, 2021. "Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technology," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22308-8
    DOI: 10.1038/s41467-021-22308-8
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    Cited by:

    1. Dinh-Huy Nguyen & Ari Chong & Yeongjin Hong & Jung-Joon Min, 2023. "Bioengineering of bacteria for cancer immunotherapy," Nature Communications, Nature, vol. 14(1), pages 1-5, December.

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