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Single-molecule long-read sequencing reveals a conserved intact long RNA profile in sperm

Author

Listed:
  • Yu H. Sun

    (University of Rochester Medical Center
    University of Rochester)

  • Anqi Wang

    (University of Iowa)

  • Chi Song

    (The Ohio State University
    Division of Reproductive Endocrinology, Geisinger Medical Center)

  • Goutham Shankar

    (University of Rochester Medical Center)

  • Rajesh K. Srivastava

    (University of Rochester Medical Center)

  • Kin Fai Au

    (University of Iowa)

  • Xin Zhiguo Li

    (University of Rochester Medical Center
    University of Rochester)

Abstract

Sperm contributes diverse RNAs to the zygote. While sperm small RNAs have been shown to impact offspring phenotypes, our knowledge of the sperm transcriptome, especially the composition of long RNAs, has been limited by the lack of sensitive, high-throughput experimental techniques that can distinguish intact RNAs from fragmented RNAs, known to abound in sperm. Here, we integrate single-molecule long-read sequencing with short-read sequencing to detect sperm intact RNAs (spiRNAs). We identify 3440 spiRNA species in mice and 4100 in humans. The spiRNA profile consists of both mRNAs and long non-coding RNAs, is evolutionarily conserved between mice and humans, and displays an enrichment in mRNAs encoding for ribosome. In sum, we characterize the landscape of intact long RNAs in sperm, paving the way for future studies on their biogenesis and functions. Our experimental and bioinformatics approaches can be applied to other tissues and organisms to detect intact transcripts.

Suggested Citation

  • Yu H. Sun & Anqi Wang & Chi Song & Goutham Shankar & Rajesh K. Srivastava & Kin Fai Au & Xin Zhiguo Li, 2021. "Single-molecule long-read sequencing reveals a conserved intact long RNA profile in sperm," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21524-6
    DOI: 10.1038/s41467-021-21524-6
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    Cited by:

    1. Yu H. Sun & Ruoqiao Huiyi Wang & Khai Du & Jiang Zhu & Jihong Zheng & Li Huitong Xie & Amanda A. Pereira & Chao Zhang & Emiliano P. Ricci & Xin Zhiguo Li, 2021. "Coupled protein synthesis and ribosome-guided piRNA processing on mRNAs," Nature Communications, Nature, vol. 12(1), pages 1-19, December.

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