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Rapid electrochemical detection of coronavirus SARS-CoV-2

Author

Listed:
  • Thanyarat Chaibun

    (Mahidol University)

  • Jiratchaya Puenpa

    (Chulalongkorn University)

  • Tatchanun Ngamdee

    (King Mongkut’s University of Technology Thonburi)

  • Nimaradee Boonapatcharoen

    (King Mongkut’s University of Technology Thonburi)

  • Pornpat Athamanolap

    (Mahidol University)

  • Anthony Peter O’Mullane

    (Queensland University of Technology (QUT))

  • Sompong Vongpunsawad

    (Chulalongkorn University)

  • Yong Poovorawan

    (Chulalongkorn University)

  • Su Yin Lee

    (AIMST University
    AIMST University)

  • Benchaporn Lertanantawong

    (Mahidol University)

Abstract

Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.

Suggested Citation

  • Thanyarat Chaibun & Jiratchaya Puenpa & Tatchanun Ngamdee & Nimaradee Boonapatcharoen & Pornpat Athamanolap & Anthony Peter O’Mullane & Sompong Vongpunsawad & Yong Poovorawan & Su Yin Lee & Benchaporn, 2021. "Rapid electrochemical detection of coronavirus SARS-CoV-2," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21121-7
    DOI: 10.1038/s41467-021-21121-7
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    Cited by:

    1. Hu Li & Huarui Gong & Tsz Hung Wong & Jingkun Zhou & Yuqiong Wang & Long Lin & Ying Dou & Huiling Jia & Xingcan Huang & Zhan Gao & Rui Shi & Ya Huang & Zhenlin Chen & Wooyoung PARK & Ji Yu Li & Hongwe, 2023. "Wireless, battery-free, multifunctional integrated bioelectronics for respiratory pathogens monitoring and severity evaluation," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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