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Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image simulation

Author

Listed:
  • Sara Sheibani

    (McGill University)

  • Kaustuv Basu

    (McGill University)

  • Ali Farnudi

    (Sharif University of Technology)

  • Aliakbar Ashkarran

    (Michigan State University)

  • Muneyoshi Ichikawa

    (McGill University
    Institute for Research in Fundamental Sciences (IPM)
    Japan Science and Technology Agency)

  • John F. Presley

    (McGill University)

  • Khanh Huy Bui

    (McGill University
    McGill University)

  • Mohammad Reza Ejtehadi

    (Sharif University of Technology)

  • Hojatollah Vali

    (McGill University
    McGill University)

  • Morteza Mahmoudi

    (Michigan State University)

Abstract

The biological identity of nanoparticles (NPs) is established by their interactions with a wide range of biomolecules around their surfaces after exposure to biological media. Understanding the true nature of the biomolecular corona (BC) in its native state is, therefore, essential for its safe and efficient application in clinical settings. The fundamental challenge is to visualize the biomolecules within the corona and their relationship/association to the surface of the NPs. Using a synergistic application of cryo-electron microscopy, cryo-electron tomography, and three-dimensional reconstruction, we revealed the unique morphological details of the biomolecules and their distribution/association with the surface of polystyrene NPs at a nanoscale resolution. The analysis of the BC at a single NP level and its variability among NPs in the same sample, and the discovery of the presence of nonspecific biomolecules in plasma residues, enable more precise characterization of NPs, improving predictions of their safety and efficacies.

Suggested Citation

  • Sara Sheibani & Kaustuv Basu & Ali Farnudi & Aliakbar Ashkarran & Muneyoshi Ichikawa & John F. Presley & Khanh Huy Bui & Mohammad Reza Ejtehadi & Hojatollah Vali & Morteza Mahmoudi, 2021. "Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image simulation," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-020-20884-9
    DOI: 10.1038/s41467-020-20884-9
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    Cited by:

    1. Didar Baimanov & Jing Wang & Jun Zhang & Ke Liu & Yalin Cong & Xiaomeng Shi & Xiaohui Zhang & Yufeng Li & Xiumin Li & Rongrong Qiao & Yuliang Zhao & Yunlong Zhou & Liming Wang & Chunying Chen, 2022. "In situ analysis of nanoparticle soft corona and dynamic evolution," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Ali Akbar Ashkarran & Hassan Gharibi & Seyed Amirhossein Sadeghi & Seyed Majed Modaresi & Qianyi Wang & Teng-Jui Lin & Ghafar Yerima & Ali Tamadon & Maryam Sayadi & Maryam Jafari & Zijin Lin & Danilo , 2024. "Small molecule modulation of protein corona for deep plasma proteome profiling," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    3. Ali Akbar Ashkarran & Hassan Gharibi & Elizabeth Voke & Markita P. Landry & Amir Ata Saei & Morteza Mahmoudi, 2022. "Measurements of heterogeneity in proteomics analysis of the nanoparticle protein corona across core facilities," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

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