IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-020-20161-9.html
   My bibliography  Save this article

Bridgin connects the outer kinetochore to centromeric chromatin

Author

Listed:
  • Shreyas Sridhar

    (Molecular Biology and Genetics Unit, Jawaharlal Nehru Center for Advanced Scientific Research (JNCASR)
    Graduate School of Frontier Biosciences, Osaka University)

  • Tetsuya Hori

    (Graduate School of Frontier Biosciences, Osaka University)

  • Reiko Nakagawa

    (RIKEN Center for Biosystems Dynamics Research (BDR))

  • Tatsuo Fukagawa

    (Graduate School of Frontier Biosciences, Osaka University)

  • Kaustuv Sanyal

    (Molecular Biology and Genetics Unit, Jawaharlal Nehru Center for Advanced Scientific Research (JNCASR)
    Graduate School of Frontier Biosciences, Osaka University)

Abstract

The microtubule-binding outer kinetochore is coupled to centromeric chromatin through CENP-CMif2, CENP-TCnn1, and CENP-UAme1 linker pathways originating from the constitutive centromere associated network (CCAN) of the inner kinetochore. Here, we demonstrate the recurrent loss of most CCAN components, including certain kinetochore linkers during the evolution of the fungal phylum of Basidiomycota. By kinetochore interactome analyses in a model basidiomycete and human pathogen Cryptococcus neoformans, a forkhead-associated domain containing protein “bridgin” was identified as a kinetochore component along with other predicted kinetochore proteins. In vivo and in vitro functional analyses of bridgin reveal its ability to connect the outer kinetochore with centromeric chromatin to ensure accurate chromosome segregation. Unlike established CCAN-based linkers, bridgin is recruited at the outer kinetochore establishing its role as a distinct family of kinetochore proteins. Presence of bridgin homologs in non-fungal lineages suggests an ancient divergent strategy exists to bridge the outer kinetochore with centromeric chromatin.

Suggested Citation

  • Shreyas Sridhar & Tetsuya Hori & Reiko Nakagawa & Tatsuo Fukagawa & Kaustuv Sanyal, 2021. "Bridgin connects the outer kinetochore to centromeric chromatin," Nature Communications, Nature, vol. 12(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-020-20161-9
    DOI: 10.1038/s41467-020-20161-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-20161-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-20161-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Yusuke Takenoshita & Masatoshi Hara & Tatsuo Fukagawa, 2022. "Recruitment of two Ndc80 complexes via the CENP-T pathway is sufficient for kinetochore functions," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    2. Priya Jaitly & Mélanie Legrand & Abhijit Das & Tejas Patel & Murielle Chauvel & Corinne Maufrais & Christophe d’Enfert & Kaustuv Sanyal, 2022. "A phylogenetically-restricted essential cell cycle progression factor in the human pathogen Candida albicans," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-020-20161-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.