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Biased localization of actin binding proteins by actin filament conformation

Author

Listed:
  • Andrew R. Harris

    (University of California, Berkeley)

  • Pamela Jreij

    (University of California, Berkeley)

  • Brian Belardi

    (University of California, Berkeley)

  • Aaron M. Joffe

    (University of California, Berkeley)

  • Andreas R. Bausch

    (Technische Universität München)

  • Daniel A. Fletcher

    (University of California, Berkeley
    Lawrence Berkeley National Laboratory
    Chan Zuckerberg Biohub)

Abstract

The assembly of actin filaments into distinct cytoskeletal structures plays a critical role in cell physiology, but how proteins localize differentially to these structures within a shared cytoplasm remains unclear. Here, we show that the actin-binding domains of accessory proteins can be sensitive to filament conformational changes. Using a combination of live cell imaging and in vitro single molecule binding measurements, we show that tandem calponin homology domains (CH1–CH2) can be mutated to preferentially bind actin networks at the front or rear of motile cells. We demonstrate that the binding kinetics of CH1–CH2 domain mutants varies as actin filament conformation is altered by perturbations that include stabilizing drugs and other binding proteins. These findings suggest that conformational changes of actin filaments in cells could help to direct accessory binding proteins to different actin cytoskeletal structures through a biophysical feedback loop.

Suggested Citation

  • Andrew R. Harris & Pamela Jreij & Brian Belardi & Aaron M. Joffe & Andreas R. Bausch & Daniel A. Fletcher, 2020. "Biased localization of actin binding proteins by actin filament conformation," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19768-9
    DOI: 10.1038/s41467-020-19768-9
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    Cited by:

    1. Qianqian Ma & Wahyu Surya & Danxia He & Hanmeng Yang & Xiao Han & Mui Hoon Nai & Chwee Teck Lim & Jaume Torres & Yansong Miao, 2024. "Spa2 remodels ADP-actin via molecular condensation under glucose starvation," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Tyler H. Ogunmowo & Haoyuan Jing & Sumana Raychaudhuri & Grant F. Kusick & Yuuta Imoto & Shuo Li & Kie Itoh & Ye Ma & Haani Jafri & Matthew B. Dalva & Edwin R. Chapman & Taekjip Ha & Shigeki Watanabe , 2023. "Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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