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Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi

Author

Listed:
  • Adriana Adolfi

    (University of California
    Liverpool School of Tropical Medicine, Vector Biology Department)

  • Valentino M. Gantz

    (Section of Cell and Developmental Biology, University of California, San Diego)

  • Nijole Jasinskiene

    (University of California)

  • Hsu-Feng Lee

    (University of California)

  • Kristy Hwang

    (University of California)

  • Gerard Terradas

    (Section of Cell and Developmental Biology, University of California, San Diego
    Tata Institute for Genetics and Society (TIGS)-UCSD)

  • Emily A. Bulger

    (Section of Cell and Developmental Biology, University of California, San Diego
    Tata Institute for Genetics and Society (TIGS)-UCSD
    Developmental and Stem Cell Biology Graduate Program, University of California
    The Gladstone Institutes)

  • Arunachalam Ramaiah

    (University of California
    Tata Institute for Genetics and Society (TIGS)-India)

  • Jared B. Bennett

    (Biophysics Graduate Group, Division of Biological Sciences, College of Letters and Science, University of California)

  • J. J. Emerson

    (University of California)

  • John M. Marshall

    (Division of Epidemiology & Biostatistics, School of Public Health, University of California
    Innovative Genomics Institute)

  • Ethan Bier

    (Section of Cell and Developmental Biology, University of California, San Diego
    Tata Institute for Genetics and Society (TIGS)-UCSD)

  • Anthony A. James

    (University of California
    University of California)

Abstract

Cas9/gRNA-mediated gene-drive systems have advanced development of genetic technologies for controlling vector-borne pathogen transmission. These technologies include population suppression approaches, genetic analogs of insecticidal techniques that reduce the number of insect vectors, and population modification (replacement/alteration) approaches, which interfere with competence to transmit pathogens. Here, we develop a recoded gene-drive rescue system for population modification of the malaria vector, Anopheles stephensi, that relieves the load in females caused by integration of the drive into the kynurenine hydroxylase gene by rescuing its function. Non-functional resistant alleles are eliminated via a dominantly-acting maternal effect combined with slower-acting standard negative selection, and rare functional resistant alleles do not prevent drive invasion. Small cage trials show that single releases of gene-drive males robustly result in efficient population modification with ≥95% of mosquitoes carrying the drive within 5-11 generations over a range of initial release ratios.

Suggested Citation

  • Adriana Adolfi & Valentino M. Gantz & Nijole Jasinskiene & Hsu-Feng Lee & Kristy Hwang & Gerard Terradas & Emily A. Bulger & Arunachalam Ramaiah & Jared B. Bennett & J. J. Emerson & John M. Marshall &, 2020. "Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19426-0
    DOI: 10.1038/s41467-020-19426-0
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    Cited by:

    1. Silvia Grilli & Roberto Galizi & Chrysanthi Taxiarchi, 2021. "Genetic Technologies for Sustainable Management of Insect Pests and Disease Vectors," Sustainability, MDPI, vol. 13(10), pages 1-19, May.

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