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Optical imaging of single-protein size, charge, mobility, and binding

Author

Listed:
  • Guangzhong Ma

    (Arizona State University)

  • Zijian Wan

    (Arizona State University
    Arizona State University)

  • Yunze Yang

    (Arizona State University)

  • Pengfei Zhang

    (Arizona State University)

  • Shaopeng Wang

    (Arizona State University)

  • Nongjian Tao

    (Arizona State University
    Arizona State University)

Abstract

Detection and identification of proteins are typically achieved by analyzing protein size, charge, mobility and binding to antibodies, which are critical for biomedical research and disease diagnosis and treatment. Despite the importance, measuring these quantities with one technology and at the single-molecule level has not been possible. Here we tether a protein to a surface with a flexible polymer, drive it into oscillation with an electric field, and image the oscillation with a near field optical imaging method, from which we determine the size, charge, and mobility of the protein. We also measure antibody binding and conformation changes in the protein. The work demonstrates a capability for comprehensive protein analysis and precision protein biomarker detection at the single molecule level.

Suggested Citation

  • Guangzhong Ma & Zijian Wan & Yunze Yang & Pengfei Zhang & Shaopeng Wang & Nongjian Tao, 2020. "Optical imaging of single-protein size, charge, mobility, and binding," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18547-w
    DOI: 10.1038/s41467-020-18547-w
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    Cited by:

    1. Pengfei Zhang & Lei Zhou & Rui Wang & Xinyu Zhou & Jiapei Jiang & Zijian Wan & Shaopeng Wang, 2022. "Evanescent scattering imaging of single protein binding kinetics and DNA conformation changes," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

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