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A cross-reactive human IgA monoclonal antibody blocks SARS-CoV-2 spike-ACE2 interaction

Author

Listed:
  • Monir Ejemel

    (MassBiologics of the University of Massachusetts Medical School)

  • Qi Li

    (MassBiologics of the University of Massachusetts Medical School)

  • Shurong Hou

    (University of Massachusetts Medical School)

  • Zachary A. Schiller

    (MassBiologics of the University of Massachusetts Medical School)

  • Julia A. Tree

    (Public Health England, Porton Down)

  • Aaron Wallace

    (MassBiologics of the University of Massachusetts Medical School)

  • Alla Amcheslavsky

    (MassBiologics of the University of Massachusetts Medical School)

  • Nese Kurt Yilmaz

    (University of Massachusetts Medical School)

  • Karen R. Buttigieg

    (Public Health England, Porton Down)

  • Michael J. Elmore

    (Public Health England, Porton Down)

  • Kerry Godwin

    (Public Health England, Porton Down)

  • Naomi Coombes

    (Public Health England, Porton Down)

  • Jacqueline R. Toomey

    (MassBiologics of the University of Massachusetts Medical School)

  • Ryan Schneider

    (MassBiologics of the University of Massachusetts Medical School)

  • Anudeep S. Ramchetty

    (MassBiologics of the University of Massachusetts Medical School)

  • Brianna J. Close

    (Boston University)

  • Da-Yuan Chen

    (Boston University)

  • Hasahn L. Conway

    (Boston University)

  • Mohsan Saeed

    (Boston University)

  • Chandrashekar Ganesa

    (MassBiologics of the University of Massachusetts Medical School)

  • Miles W. Carroll

    (Public Health England, Porton Down)

  • Lisa A. Cavacini

    (MassBiologics of the University of Massachusetts Medical School)

  • Mark S. Klempner

    (MassBiologics of the University of Massachusetts Medical School)

  • Celia A. Schiffer

    (University of Massachusetts Medical School)

  • Yang Wang

    (MassBiologics of the University of Massachusetts Medical School)

Abstract

COVID-19 caused by SARS-CoV-2 has become a global pandemic requiring the development of interventions for the prevention or treatment to curtail mortality and morbidity. No vaccine to boost mucosal immunity, or as a therapeutic, has yet been developed to SARS-CoV-2. In this study, we discover and characterize a cross-reactive human IgA monoclonal antibody, MAb362. MAb362 binds to both SARS-CoV and SARS-CoV-2 spike proteins and competitively blocks ACE2 receptor binding, by overlapping the ACE2 structural binding epitope. Furthermore, MAb362 IgA neutralizes both pseudotyped SARS-CoV and SARS-CoV-2 in 293 cells expressing ACE2. When converted to secretory IgA, MAb326 also neutralizes authentic SARS-CoV-2 virus while the IgG isotype shows no neutralization. Our results suggest that SARS-CoV-2 specific IgA antibodies, such as MAb362, may provide effective immunity against SARS-CoV-2 by inducing mucosal immunity within the respiratory system, a potentially critical feature of an effective vaccine.

Suggested Citation

  • Monir Ejemel & Qi Li & Shurong Hou & Zachary A. Schiller & Julia A. Tree & Aaron Wallace & Alla Amcheslavsky & Nese Kurt Yilmaz & Karen R. Buttigieg & Michael J. Elmore & Kerry Godwin & Naomi Coombes , 2020. "A cross-reactive human IgA monoclonal antibody blocks SARS-CoV-2 spike-ACE2 interaction," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18058-8
    DOI: 10.1038/s41467-020-18058-8
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    Cited by:

    1. Karla P. Garcia-Pelagio & Tamara Hew-Butler & Mariane M. Fahlman & Joseph A. Roche, 2021. "Women’s Lives Matter—The Critical Need for Women to Prioritize Optimal Physical Activity to Reduce COVID-19 Illness Risk and Severity," IJERPH, MDPI, vol. 18(19), pages 1-20, September.
    2. Zhenzhen Wang & Shiqi Hu & Kristen D. Popowski & Shuo Liu & Dashuai Zhu & Xuan Mei & Junlang Li & Yilan Hu & Phuong-Uyen C. Dinh & Xiaojie Wang & Ke Cheng, 2024. "Inhalation of ACE2-expressing lung exosomes provides prophylactic protection against SARS-CoV-2," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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