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Single-cell analysis of two severe COVID-19 patients reveals a monocyte-associated and tocilizumab-responding cytokine storm

Author

Listed:
  • Chuang Guo

    (University of Science and Technology of China)

  • Bin Li

    (University of Science and Technology of China)

  • Huan Ma

    (University of Science and Technology of China)

  • Xiaofang Wang

    (University of Science and Technology of China)

  • Pengfei Cai

    (University of Science and Technology of China)

  • Qiaoni Yu

    (University of Science and Technology of China)

  • Lin Zhu

    (University of Science and Technology of China)

  • Liying Jin

    (University of Science and Technology of China)

  • Chen Jiang

    (University of Science and Technology of China)

  • Jingwen Fang

    (HanGene Biotech, Xiaoshan Innovation Polis)

  • Qian Liu

    (University of Science and Technology of China)

  • Dandan Zong

    (University of Science and Technology of China)

  • Wen Zhang

    (University of Science and Technology of China)

  • Yichen Lu

    (University of Science and Technology of China)

  • Kun Li

    (University of Science and Technology of China)

  • Xuyuan Gao

    (University of Science and Technology of China)

  • Binqing Fu

    (University of Science and Technology of China
    CAS Center for Excellence in Molecular Cell Sciences, the CAS Key Laboratory of Innate Immunity and Chronic Disease, University of Science and Technology of China)

  • Lianxin Liu

    (University of Science and Technology of China)

  • Xiaoling Ma

    (University of Science and Technology of China)

  • Jianping Weng

    (University of Science and Technology of China)

  • Haiming Wei

    (University of Science and Technology of China
    CAS Center for Excellence in Molecular Cell Sciences, the CAS Key Laboratory of Innate Immunity and Chronic Disease, University of Science and Technology of China)

  • Tengchuan Jin

    (University of Science and Technology of China
    CAS Center for Excellence in Molecular Cell Sciences, the CAS Key Laboratory of Innate Immunity and Chronic Disease, University of Science and Technology of China)

  • Jun Lin

    (University of Science and Technology of China
    CAS Center for Excellence in Molecular Cell Sciences, the CAS Key Laboratory of Innate Immunity and Chronic Disease, University of Science and Technology of China)

  • Kun Qu

    (University of Science and Technology of China
    CAS Center for Excellence in Molecular Cell Sciences, the CAS Key Laboratory of Innate Immunity and Chronic Disease, University of Science and Technology of China
    University of Science and Technology of China)

Abstract

Several studies show that the immunosuppressive drugs targeting the interleukin-6 (IL-6) receptor, including tocilizumab, ameliorate lethal inflammatory responses in COVID-19 patients infected with SARS-CoV-2. Here, by employing single-cell analysis of the immune cell composition of two severe-stage COVID-19 patients prior to and following tocilizumab-induced remission, we identify a monocyte subpopulation that contributes to the inflammatory cytokine storms. Furthermore, although tocilizumab treatment attenuates the inflammation, immune cells, including plasma B cells and CD8+ T cells, still exhibit robust humoral and cellular antiviral immune responses. Thus, in addition to providing a high-dimensional dataset on the immune cell distribution at multiple stages of the COVID-19, our work also provides insights into the therapeutic effects of tocilizumab, and identifies potential target cell populations for treating COVID-19-related cytokine storms.

Suggested Citation

  • Chuang Guo & Bin Li & Huan Ma & Xiaofang Wang & Pengfei Cai & Qiaoni Yu & Lin Zhu & Liying Jin & Chen Jiang & Jingwen Fang & Qian Liu & Dandan Zong & Wen Zhang & Yichen Lu & Kun Li & Xuyuan Gao & Binq, 2020. "Single-cell analysis of two severe COVID-19 patients reveals a monocyte-associated and tocilizumab-responding cytokine storm," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17834-w
    DOI: 10.1038/s41467-020-17834-w
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    Cited by:

    1. Junedh M. Amrute & Alexandra M. Perry & Gautam Anand & Carlos Cruchaga & Karl G. Hock & Christopher W. Farnsworth & Gwendalyn J. Randolph & Kory J. Lavine & Ashley L. Steed, 2022. "Cell specific peripheral immune responses predict survival in critical COVID-19 patients," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Jonathan D. Herman & Chuangqi Wang & Carolin Loos & Hyunah Yoon & Johanna Rivera & M. Eugenia Dieterle & Denise Haslwanter & Rohit K. Jangra & Robert H. Bortz & Katharine J. Bar & Boris Julg & Kartik , 2021. "Functional convalescent plasma antibodies and pre-infusion titers shape the early severe COVID-19 immune response," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    3. Lei Xiong & Kang Tian & Yuzhe Li & Weixi Ning & Xin Gao & Qiangfeng Cliff Zhang, 2022. "Online single-cell data integration through projecting heterogeneous datasets into a common cell-embedding space," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

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