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Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems

Author

Listed:
  • Hajime Shigemitsu

    (Graduate School of Engineering, Kyoto University, Nishikyo-ku
    Graduate School of Engineering, Osaka University)

  • Ryou Kubota

    (Graduate School of Engineering, Kyoto University, Nishikyo-ku)

  • Keisuke Nakamura

    (Graduate School of Engineering, Kyoto University, Nishikyo-ku)

  • Tomonobu Matsuzaki

    (Graduate School of Engineering, Kyoto University, Nishikyo-ku)

  • Saori Minami

    (Kyoto Institute of Technology)

  • Takuma Aoyama

    (Kyoto Institute of Technology)

  • Kenji Urayama

    (Kyoto Institute of Technology)

  • Itaru Hamachi

    (Graduate School of Engineering, Kyoto University, Nishikyo-ku
    JST-ERATO, Hamachi Innovative Molecular Technology for Neuroscience, Kyoto University)

Abstract

Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases. Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies. Here, we describe a supramolecular/agarose hydrogel composite that can release a protein in response to a non-enzymatic protein. A non-enzymatic protein-responsive system is developed by hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set. In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively. Integrating the enzyme activation set with the composite allows for controlled release of the embedded RNase in response to an antibody. Such composite hydrogels would be promising as a matrix embedded in a body, which can autonomously release biopharmaceuticals by sensing biomarker proteins.

Suggested Citation

  • Hajime Shigemitsu & Ryou Kubota & Keisuke Nakamura & Tomonobu Matsuzaki & Saori Minami & Takuma Aoyama & Kenji Urayama & Itaru Hamachi, 2020. "Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17698-0
    DOI: 10.1038/s41467-020-17698-0
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    Cited by:

    1. Keisuke Nakamura & Ryou Kubota & Takuma Aoyama & Kenji Urayama & Itaru Hamachi, 2023. "Four distinct network patterns of supramolecular/polymer composite hydrogels controlled by formation kinetics and interfiber interactions," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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