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PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria

Author

Listed:
  • Yael Elbaz-Alon

    (University of California Davis
    Weizmann Institute of Science)

  • Yuting Guo

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Nadav Segev

    (Weizmann Institute of Science)

  • Michal Harel

    (Tel-Aviv University)

  • Daniel E. Quinnell

    (University of California Davis)

  • Tamar Geiger

    (Tel-Aviv University)

  • Ori Avinoam

    (Weizmann Institute of Science)

  • Dong Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Jodi Nunnari

    (University of California Davis)

Abstract

Endosomes are compositionally dynamic organelles that regulate signaling, nutrient status and organelle quality by specifying whether material entering the cells will be shuttled back to the cell surface or degraded by the lysosome. Recently, membrane contact sites (MCSs) between the endoplasmic reticulum (ER) and endosomes have emerged as important players in endosomal protein sorting, dynamics and motility. Here, we show that PDZD8, a Synaptotagmin-like Mitochondrial lipid-binding Proteins (SMP) domain-containing ER transmembrane protein, utilizes distinct domains to interact with Rab7-GTP and the ER transmembrane protein Protrudin and together these components localize to an ER-late endosome MCS. At these ER-late endosome MCSs, mitochondria are also recruited to form a three-way contact. Thus, our data indicate that PDZD8 is a shared component of two distinct MCSs and suggest a role for SMP-mediated lipid transport in the regulation of endosome function.

Suggested Citation

  • Yael Elbaz-Alon & Yuting Guo & Nadav Segev & Michal Harel & Daniel E. Quinnell & Tamar Geiger & Ori Avinoam & Dong Li & Jodi Nunnari, 2020. "PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17451-7
    DOI: 10.1038/s41467-020-17451-7
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    Cited by:

    1. Yunyun Wang & Zhenni Li & Xinyu Wang & Ziyuan Zhao & Li Jiao & Ruming Liu & Keying Wang & Rui Ma & Yang Yang & Guo Chen & Yong Wang & Xin Bian, 2023. "Insights into membrane association of the SMP domain of extended synaptotagmin," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Darshini Jeyasimman & Bilge Ercan & Dennis Dharmawan & Tomoki Naito & Jingbo Sun & Yasunori Saheki, 2021. "PDZD-8 and TEX-2 regulate endosomal PI(4,5)P2 homeostasis via lipid transport to promote embryogenesis in C. elegans," Nature Communications, Nature, vol. 12(1), pages 1-21, December.

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