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Two linear epitopes on the SARS-CoV-2 spike protein that elicit neutralising antibodies in COVID-19 patients

Author

Listed:
  • Chek Meng Poh

    (Technology and Research, Immunos, Biopolis)

  • Guillaume Carissimo

    (Technology and Research, Immunos, Biopolis)

  • Bei Wang

    (Technology and Research, Immunos, Biopolis)

  • Siti Naqiah Amrun

    (Technology and Research, Immunos, Biopolis)

  • Cheryl Yi-Pin Lee

    (Technology and Research, Immunos, Biopolis)

  • Rhonda Sin-Ling Chee

    (Technology and Research, Immunos, Biopolis)

  • Siew-Wai Fong

    (Technology and Research, Immunos, Biopolis
    National University of Singapore)

  • Nicholas Kim-Wah Yeo

    (Technology and Research, Immunos, Biopolis)

  • Wen-Hsin Lee

    (Technology and Research, Immunos, Biopolis)

  • Anthony Torres-Ruesta

    (Technology and Research, Immunos, Biopolis
    National University of Singapore)

  • Yee-Sin Leo

    (16 Jalan Tan Tock Seng
    Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng
    Nanyang Technological University
    National University of Singapore and National University Health System)

  • Mark I-Cheng Chen

    (16 Jalan Tan Tock Seng
    National University of Singapore and National University Health System)

  • Seow-Yen Tan

    (Changi General Hospital)

  • Louis Yi Ann Chai

    (Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng
    National University Hospital)

  • Shirin Kalimuddin

    (Singapore General Hospital
    Duke-NUS Medical School)

  • Shirley Seah Gek Kheng

    (DSO National Laboratories)

  • Siew-Yee Thien

    (Singapore General Hospital)

  • Barnaby Edward Young

    (16 Jalan Tan Tock Seng
    Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng
    Nanyang Technological University)

  • David C. Lye

    (16 Jalan Tan Tock Seng
    Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng
    Nanyang Technological University
    National University of Singapore and National University Health System)

  • Brendon John Hanson

    (DSO National Laboratories)

  • Cheng-I Wang

    (Technology and Research, Immunos, Biopolis)

  • Laurent Renia

    (Technology and Research, Immunos, Biopolis)

  • Lisa F. P. Ng

    (Technology and Research, Immunos, Biopolis
    National University of Singapore
    University of Liverpool, Liverpool)

Abstract

Given the ongoing SARS-CoV-2 pandemic, identification of immunogenic targets against the coronavirus spike glycoprotein will provide crucial advances towards the development of sensitive diagnostic tools and potential vaccine candidate targets. In this study, using pools of overlapping linear B-cell peptides, we report two IgG immunodominant regions on SARS-CoV-2 spike glycoprotein that are recognised by sera from COVID-19 convalescent patients. Notably, one is specific to SARS-CoV-2, which is located in close proximity to the receptor binding domain. The other region, which is localised at the fusion peptide, could potentially function as a pan-SARS target. Functionally, antibody depletion assays demonstrate that antibodies targeting these immunodominant regions significantly alter virus neutralisation capacities. Taken together, identification and validation of these neutralising B-cell epitopes will provide insights towards the design of diagnostics and vaccine candidates against this high priority coronavirus.

Suggested Citation

  • Chek Meng Poh & Guillaume Carissimo & Bei Wang & Siti Naqiah Amrun & Cheryl Yi-Pin Lee & Rhonda Sin-Ling Chee & Siew-Wai Fong & Nicholas Kim-Wah Yeo & Wen-Hsin Lee & Anthony Torres-Ruesta & Yee-Sin Le, 2020. "Two linear epitopes on the SARS-CoV-2 spike protein that elicit neutralising antibodies in COVID-19 patients," Nature Communications, Nature, vol. 11(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16638-2
    DOI: 10.1038/s41467-020-16638-2
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    Cited by:

    1. Iris N. Pardieck & Tetje C. van der Sluis & Esmé T. I. van der Gracht & Dominique M. B. Veerkamp & Felix M. Behr & Suzanne van Duikeren & Guillaume Beyrend & Jasper Rip & Reza Nadafi & Elham Beyranvan, 2022. "A third vaccination with a single T cell epitope confers protection in a murine model of SARS-CoV-2 infection," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Laurent Renia & Yun Shan Goh & Angeline Rouers & Nina Bert & Wan Ni Chia & Jean-Marc Chavatte & Siew‐Wai Fong & Zi Wei Chang & Nicole Ziyi Zhuo & Matthew Zirui Tay & Yi-Hao Chan & Chee Wah Tan & Nicho, 2022. "Lower vaccine-acquired immunity in the elderly population following two-dose BNT162b2 vaccination is alleviated by a third vaccine dose," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    3. Anahita Fathi & Christine Dahlke & Verena Krähling & Alexandra Kupke & Nisreen M. A. Okba & Matthijs P. Raadsen & Jasmin Heidepriem & Marcel A. Müller & Grigori Paris & Susan Lassen & Michael Klüver &, 2022. "Increased neutralization and IgG epitope identification after MVA-MERS-S booster vaccination against Middle East respiratory syndrome," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    4. Caitlin I. Stoddard & Kevin Sung & Zak A. Yaffe & Haidyn Weight & Guillaume Beaudoin-Bussières & Jared Galloway & Soren Gantt & Judith Adhiambo & Emily R. Begnel & Ednah Ojee & Jennifer Slyker & Dalto, 2023. "Elevated binding and functional antibody responses to SARS-CoV-2 in infants versus mothers," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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