IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-15548-7.html
   My bibliography  Save this article

Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages

Author

Listed:
  • Jennifer M. Rosenbluth

    (Harvard Medical School)

  • Ron C. J. Schackmann

    (Harvard Medical School)

  • G. Kenneth Gray

    (Harvard Medical School)

  • Laura M. Selfors

    (Harvard Medical School)

  • Carman Man-Chung Li

    (Harvard Medical School)

  • Mackenzie Boedicker

    (Harvard Medical School)

  • Hendrik J. Kuiken

    (Harvard Medical School)

  • Andrea Richardson

    (Brigham & Women’s Hospital)

  • Jane Brock

    (Brigham & Women’s Hospital)

  • Judy Garber

    (Dana-Farber Cancer Institute)

  • Deborah Dillon

    (Brigham & Women’s Hospital)

  • Norman Sachs

    (Hubrecht Institute)

  • Hans Clevers

    (Hubrecht Institute)

  • Joan S. Brugge

    (Harvard Medical School)

Abstract

Recently, organoid technology has been used to generate a large repository of breast cancer organoids. Here we present an extensive evaluation of the ability of organoid culture technology to preserve complex stem/progenitor and differentiated cell types via long-term propagation of normal human mammary tissues. Basal/stem and luminal progenitor cells can differentiate in culture to generate mature basal and luminal cell types, including ER+ cells that have been challenging to maintain in culture. Cells associated with increased cancer risk can also be propagated. Single-cell analyses of matched organoid cultures and native tissues by mass cytometry for 38 markers provide a higher resolution representation of the multiple mammary epithelial cell types in the organoids, and demonstrate that protein expression patterns of the tissue of origin can be preserved in culture. These studies indicate that organoid cultures provide a valuable platform for studies of mammary differentiation, transformation, and breast cancer risk.

Suggested Citation

  • Jennifer M. Rosenbluth & Ron C. J. Schackmann & G. Kenneth Gray & Laura M. Selfors & Carman Man-Chung Li & Mackenzie Boedicker & Hendrik J. Kuiken & Andrea Richardson & Jane Brock & Judy Garber & Debo, 2020. "Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15548-7
    DOI: 10.1038/s41467-020-15548-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-15548-7
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-15548-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Maryam Ghaderi Najafabadi & G. Kenneth Gray & Li Ren Kong & Komal Gupta & David Perera & Huw Naylor & Joan S. Brugge & Ashok R. Venkitaraman & Mona Shehata, 2023. "A transcriptional response to replication stress selectively expands a subset of Brca2-mutant mammary epithelial cells," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Maria Fankhaenel & Farahnaz S. Golestan Hashemi & Larissa Mourao & Emily Lucas & Manal M. Hosawi & Paul Skipp & Xavier Morin & Colinda L.G.J. Scheele & Salah Elias, 2023. "Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    3. Yeo-Jun Yoon & Donghyun Kim & Kwon Yong Tak & Seungyeon Hwang & Jisun Kim & Nam Suk Sim & Jae-Min Cho & Dojin Choi & Yongmi Ji & Junho K. Hur & Hyunki Kim & Jong-Eun Park & Jae-Yol Lim, 2022. "Salivary gland organoid culture maintains distinct glandular properties of murine and human major salivary glands," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15548-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.