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The RNA-binding protein DAZL functions as repressor and activator of mRNA translation during oocyte maturation

Author

Listed:
  • Cai-Rong Yang

    (University of California
    University of California
    University of California)

  • Gabriel Rajkovic

    (University of California
    University of California
    University of California)

  • Enrico Maria Daldello

    (University of California
    University of California
    University of California)

  • Xuan G. Luong

    (University of California
    University of California
    University of California)

  • Jing Chen

    (University of California
    University of California
    University of California)

  • Marco Conti

    (University of California
    University of California
    University of California)

Abstract

Deleted in azoospermia-like (DAZL) is an RNA-binding protein critical for gamete development. In full-grown oocytes, the DAZL protein increases 4-fold during reentry into the meiotic cell cycle. Here, we have investigated the functional significance of this accumulation at a genome-wide level. Depletion of DAZL causes a block in maturation and widespread disruption in the pattern of ribosome loading on maternal transcripts. In addition to decreased translation, DAZL depletion also causes translational activation of a distinct subset of mRNAs both in quiescent and maturing oocytes, a function recapitulated with YFP-3′UTR reporters. DAZL binds to mRNAs whose translation is both repressed and activated during maturation. Injection of recombinant DAZL protein in DAZL-depleted oocytes rescues the translation and maturation to MII. Mutagenesis of putative DAZL-binding sites in these mRNAs mimics the effect of DAZL depletion. These findings demonstrate that DAZL regulates translation of maternal mRNAs, functioning both as the translational repressor and activator during oocyte maturation.

Suggested Citation

  • Cai-Rong Yang & Gabriel Rajkovic & Enrico Maria Daldello & Xuan G. Luong & Jing Chen & Marco Conti, 2020. "The RNA-binding protein DAZL functions as repressor and activator of mRNA translation during oocyte maturation," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15209-9
    DOI: 10.1038/s41467-020-15209-9
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    Cited by:

    1. Gyu Ik Jung & Daniela Londoño-Vásquez & Sungjin Park & Ahna R. Skop & Ahmed Z. Balboula & Karen Schindler, 2023. "An oocyte meiotic midbody cap is required for developmental competence in mice," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Wenqi Hu & Haitao Zeng & Yanan Shi & Chuanchuan Zhou & Jiana Huang & Lei Jia & Siqi Xu & Xiaoyu Feng & Yanyan Zeng & Tuanlin Xiong & Wenze Huang & Peng Sun & Yajie Chang & Tingting Li & Cong Fang & Ke, 2022. "Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturation," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

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