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Probing the physical limits of reliable DNA data retrieval

Author

Listed:
  • Lee Organick

    (University of Washington)

  • Yuan-Jyue Chen

    (Microsoft)

  • Siena Dumas Ang

    (Microsoft)

  • Randolph Lopez

    (University of Washington)

  • Xiaomeng Liu

    (University of Washington)

  • Karin Strauss

    (Microsoft)

  • Luis Ceze

    (University of Washington)

Abstract

Synthetic DNA is gaining momentum as a potential storage medium for archival data storage. In this process, digital information is translated into sequences of nucleotides and the resulting synthetic DNA strands are then stored for later retrieval. Here, we demonstrate reliable file recovery with PCR-based random access when as few as ten copies per sequence are stored, on average. This results in density of about 17 exabytes/gram, nearly two orders of magnitude greater than prior work has shown. We successfully retrieve the same data in a complex pool of over 1010 unique sequences per microliter with no evidence that we have begun to approach complexity limits. Finally, we also investigate the effects of file size and sequencing coverage on successful file retrieval and look for systematic DNA strand drop out. These findings substantiate the robustness and high data density of the process examined here.

Suggested Citation

  • Lee Organick & Yuan-Jyue Chen & Siena Dumas Ang & Randolph Lopez & Xiaomeng Liu & Karin Strauss & Luis Ceze, 2020. "Probing the physical limits of reliable DNA data retrieval," Nature Communications, Nature, vol. 11(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14319-8
    DOI: 10.1038/s41467-020-14319-8
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    Cited by:

    1. Jiongyu Zhang & Chengyu Hou & Changchun Liu, 2024. "CRISPR-powered quantitative keyword search engine in DNA data storage," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Afsaneh Sadremomtaz & Robert F. Glass & Jorge Eduardo Guerrero & Dennis R. LaJeunesse & Eric A. Josephs & Reza Zadegan, 2023. "Digital data storage on DNA tape using CRISPR base editors," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    3. Christopher N. Takahashi & David P. Ward & Carlo Cazzaniga & Christopher Frost & Paolo Rech & Kumkum Ganguly & Sean Blanchard & Steve Wender & Bichlien H. Nguyen & Jake A. Smith, 2024. "Evaluating the risk of data loss due to particle radiation damage in a DNA data storage system," Nature Communications, Nature, vol. 15(1), pages 1-9, December.

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