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Ionophore constructed from non-covalent assembly of a G-quadruplex and liponucleoside transports K+-ion across biological membranes

Author

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  • Manish Debnath

    (Indian Association for the Cultivation of Science, Jadavpur)

  • Sandipan Chakraborty

    (Indian Association for the Cultivation of Science, Jadavpur)

  • Y. Pavan Kumar

    (Indian Association for the Cultivation of Science, Jadavpur)

  • Ritapa Chaudhuri

    (Indian Association for the Cultivation of Science, Jadavpur)

  • Biman Jana

    (Indian Association for the Cultivation of Science, Jadavpur)

  • Jyotirmayee Dash

    (Indian Association for the Cultivation of Science, Jadavpur)

Abstract

The selective transport of ions across cell membranes, controlled by membrane proteins, is critical for a living organism. DNA-based systems have emerged as promising artificial ion transporters. However, the development of stable and selective artificial ion transporters remains a formidable task. We herein delineate the construction of an artificial ionophore using a telomeric DNA G-quadruplex (h-TELO) and a lipophilic guanosine (MG). MG stabilizes h-TELO by non-covalent interactions and, along with the lipophilic side chain, promotes the insertion of h-TELO within the hydrophobic lipid membrane. Fluorescence assays, electrophysiology measurements and molecular dynamics simulations reveal that MG/h-TELO preferentially transports K+-ions in a stimuli-responsive manner. The preferential K+-ion transport is presumably due to conformational changes of the ionophore in response to different ions. Moreover, the ionophore transports K+-ions across CHO and K-562 cell membranes. This study may serve as a design principle to generate selective DNA-based artificial transporters for therapeutic applications.

Suggested Citation

  • Manish Debnath & Sandipan Chakraborty & Y. Pavan Kumar & Ritapa Chaudhuri & Biman Jana & Jyotirmayee Dash, 2020. "Ionophore constructed from non-covalent assembly of a G-quadruplex and liponucleoside transports K+-ion across biological membranes," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-13834-7
    DOI: 10.1038/s41467-019-13834-7
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    Cited by:

    1. Hui Chen & Shaohong Zhou & Kleins Ngocho & Jing Zheng & Xiaoxiao He & Jin Huang & Kemin Wang & Hui Shi & Jianbo Liu, 2024. "Oriented triplex DNA as a synthetic receptor for transmembrane signal transduction," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Jie Shen & Yongting Gu & Lingjie Ke & Qiuping Zhang & Yin Cao & Yuchao Lin & Zhen Wu & Caisheng Wu & Yuguang Mu & Yun-Long Wu & Changliang Ren & Huaqiang Zeng, 2022. "Cholesterol-stabilized membrane-active nanopores with anticancer activities," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    3. Raj Paul & Debasish Dutta & Titas Kumar Mukhopadhyay & Diana Müller & Binayak Lala & Ayan Datta & Harald Schwalbe & Jyotirmayee Dash, 2024. "A non-B DNA binding peptidomimetic channel alters cellular functions," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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