IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-019-13834-7.html
   My bibliography  Save this article

Ionophore constructed from non-covalent assembly of a G-quadruplex and liponucleoside transports K+-ion across biological membranes

Author

Listed:
  • Manish Debnath

    (Indian Association for the Cultivation of Science, Jadavpur)

  • Sandipan Chakraborty

    (Indian Association for the Cultivation of Science, Jadavpur)

  • Y. Pavan Kumar

    (Indian Association for the Cultivation of Science, Jadavpur)

  • Ritapa Chaudhuri

    (Indian Association for the Cultivation of Science, Jadavpur)

  • Biman Jana

    (Indian Association for the Cultivation of Science, Jadavpur)

  • Jyotirmayee Dash

    (Indian Association for the Cultivation of Science, Jadavpur)

Abstract

The selective transport of ions across cell membranes, controlled by membrane proteins, is critical for a living organism. DNA-based systems have emerged as promising artificial ion transporters. However, the development of stable and selective artificial ion transporters remains a formidable task. We herein delineate the construction of an artificial ionophore using a telomeric DNA G-quadruplex (h-TELO) and a lipophilic guanosine (MG). MG stabilizes h-TELO by non-covalent interactions and, along with the lipophilic side chain, promotes the insertion of h-TELO within the hydrophobic lipid membrane. Fluorescence assays, electrophysiology measurements and molecular dynamics simulations reveal that MG/h-TELO preferentially transports K+-ions in a stimuli-responsive manner. The preferential K+-ion transport is presumably due to conformational changes of the ionophore in response to different ions. Moreover, the ionophore transports K+-ions across CHO and K-562 cell membranes. This study may serve as a design principle to generate selective DNA-based artificial transporters for therapeutic applications.

Suggested Citation

  • Manish Debnath & Sandipan Chakraborty & Y. Pavan Kumar & Ritapa Chaudhuri & Biman Jana & Jyotirmayee Dash, 2020. "Ionophore constructed from non-covalent assembly of a G-quadruplex and liponucleoside transports K+-ion across biological membranes," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-13834-7
    DOI: 10.1038/s41467-019-13834-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-13834-7
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-13834-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jie Shen & Yongting Gu & Lingjie Ke & Qiuping Zhang & Yin Cao & Yuchao Lin & Zhen Wu & Caisheng Wu & Yuguang Mu & Yun-Long Wu & Changliang Ren & Huaqiang Zeng, 2022. "Cholesterol-stabilized membrane-active nanopores with anticancer activities," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    2. Raj Paul & Debasish Dutta & Titas Kumar Mukhopadhyay & Diana Müller & Binayak Lala & Ayan Datta & Harald Schwalbe & Jyotirmayee Dash, 2024. "A non-B DNA binding peptidomimetic channel alters cellular functions," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-13834-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.